S-Space College of Agriculture and Life Sciences (농업생명과학대학) Dept. of Food and Animal Biotechnology (식품·동물생명공학부) Journal Papers (저널논문_식품·동물생명공학부)
Development and characterization of a recombinant chicken single-chain Fv antibody detecting Eimeria acervulina sporozoite antigen
- Park, Kyung Je; Park, Dong Woon; Kim, Chun Hee; Han, Beom Ku; Park, Tae Sub; Han, Jae Yong; Lillehoj, Hyun Soon; Kim, Jin-Kyoo
- Issue Date
- Biotechnology Letters, vol.27 no.5, pp. 289-295
- Chicken monoclonal antibody (mAb), 8C3, which is reactive with a sporozoite antigen of Eimeria acervulina, is a potential therapeutic agent against avian coccidiosis caused by Eimeria spp. However, production of large amounts of 8C3 mAb in cell culture system is labor intensive and not cost-effective. Accordingly, recombinant single chain variable fragment (ScFv) antibody was constructed by amplification of the VH and VL genes from chicken hybridoma, 8C3 and when expressed in E. coli gave 5 mg l)1. The expressed protein showed antigen binding activity equivalent to that of the parental mAb. In addition, nucleotide sequence comparison of 8C3 gene to the germline chicken VL genes suggested that the gene conversion with Vk pseudogenes might contribute to the diversification of VL genes in chickens.
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