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Development and characterization of a recombinant chicken single-chain Fv antibody detecting Eimeria acervulina sporozoite antigen

Cited 12 time in Web of Science Cited 19 time in Scopus
Authors
Park, Kyung Je; Park, Dong Woon; Kim, Chun Hee; Han, Beom Ku; Park, Tae Sub; Han, Jae Yong; Lillehoj, Hyun Soon; Kim, Jin-Kyoo
Issue Date
2005
Publisher
Springer
Citation
Biotechnology Letters, vol.27 no.5, pp. 289-295
Keywords
chicken monoclonal antibodyEimeria acervulinagene conversionrecombinant ScFv
Abstract
Chicken monoclonal antibody (mAb), 8C3, which is reactive with a sporozoite antigen of Eimeria acervulina, is a potential therapeutic agent against avian coccidiosis caused by Eimeria spp. However, production of large amounts of 8C3 mAb in cell culture system is labor intensive and not cost-effective. Accordingly, recombinant single chain variable fragment (ScFv) antibody was constructed by amplification of the VH and VL genes from chicken hybridoma, 8C3 and when expressed in E. coli gave 5 mg l)1. The expressed protein showed antigen binding activity equivalent to that of the parental mAb. In addition, nucleotide sequence comparison of 8C3 gene to the germline chicken VL genes suggested that the gene conversion with Vk pseudogenes might contribute to the diversification of VL genes in chickens.
ISSN
0141-5492
Language
English
URI
http://hdl.handle.net/10371/100206
DOI
https://doi.org/10.1007/s10529-005-0682-8
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
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