Browse

Production of transgenic cloned piglets from genetically transformed fetal fibroblasts selected by green fluorescent protein

Cited 61 time in Web of Science Cited 69 time in Scopus
Authors
Lee, Gab Sang; Kim, Hye Soo; Hyun, Sang Hwan; Lee, So Hyun; Jeon, Hyun Yong; Nam, Dong Hyun; Jeong, Yeon Woo; Kim, Sue; Kim, Ji Hye; Han, Jae Yong; Ahn, Curie; Kang, Sung Keun; Lee, Byeong Chun; Hwang, Woo Suk
Issue Date
2005
Publisher
Elsevier
Citation
Theriogenology, vol.63 no.4, pp. 973-991
Keywords
PorcineEmbryosTransgenicEPONuclear transferTopic category formReproductive technologies
Abstract
This study was performed to develop a system for porcine somatic cell nuclear transfer (SCNT) and to produce human erythropoietin (hEPO)-transgenic cloned piglets. Porcine fetal fibroblasts were transfected with an expression plasmid (phEPO-GFP). In Experiment 1, the effect of transfection of phEPO-GFP transgene on development of porcine SCNT embryos was investigated. Three fetal fibroblast cell lines (two male and one female) with or without transfected with phEPO-GFP trasngene were used as donor cells for SCNT. Lower fusion rates were observed in two lines of transfected cells as compared to those of the control cells. In Experiment 2, the effect was examined of elevated Ca2+ concentration in the fusion/activation medium on development of transfected SCNT embryos. The rates of fusion and blastocyst formation were significantly increased by supplementing 1.0 mMof CaCl2 (versus 0.1 mM) into the fusion/activation medium. In Experiment 3, the effect was studied of a chemical treatment (cytochalasin B) after electric fusion/activation (F/A) on porcine transgenic SCNT embryo development. The electric F/A + cytochalasin B treatment increased total cell number in blastocysts as compared to that of electric F/A treatment alone. In Experiment 4, transgenic cloned embryos were transferred to surrogate mothers and a total of six cloned piglets were born. Transgenic cloned piglets were confirmed by polymerase chain reaction and Southern blot analysis. From a single surrogate mother, female and male transgenic cloned piglets were produced by transferring pooled SCNT embryos derived from female and male transfected donor cells. In conclusion, a system for porcine SCNT was developed and led to the successful production of hEPO transgenic cloned piglets.
ISSN
0093-691X
Language
English
URI
http://hdl.handle.net/10371/100208
DOI
https://doi.org/10.1016/j.theriogenology.2004.04.017
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse