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Proteome analysis of chicken embryonic gonads: Identification of major proteins from cultured gonadal primordial germ cells

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dc.contributor.authorHan, Beom Ku-
dc.contributor.authorKim, Jin Nam-
dc.contributor.authorShin, Ji Hye-
dc.contributor.authorKim, Jin-Kyoo-
dc.contributor.authorJo, Do Hyun-
dc.contributor.authorKim, Heebal-
dc.contributor.authorHan, Jae Yong-
dc.date.accessioned2017-01-23T08:03:11Z-
dc.date.available2017-01-23T08:03:11Z-
dc.date.issued2005-
dc.identifier.citationMolecular Reproduction and Development, vol.72 no.4, pp. 521-529ko_KR
dc.identifier.issn1040-452X-
dc.identifier.urihttp://hdl.handle.net/10371/100219-
dc.description.abstractThe domestic chicken (Gallus gallus) is an important model for research in developmental biology because its embryonic development occurs in ovo. To examine the mechanism of embryonic germ cell development, we constructed proteome map of gonadal primordial germ cells (gPGCs) from chicken embryonic gonads. Embryonic gonads were collected from 500 embryos at 6 days of incubation, and the gPGCs were cultured in vitro until colony formed. After 7–10 days in culture, gPGC colonies were separated from gonadal stroma cells (GSCs). Soluble extracts of cultured gPGCs were then fractionated by two-dimensional gel electrophoresis (pH 4–7). A number of protein spots, including those that displayed significant expression levels, were then identified by use of matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry and LC-MS/ MS. Of the 89 gPGC spots examined, 50 yielded mass spectra that matched avian proteins found in on-line databases. Proteome map of this type will serve as an important reference for germ cell biology and transgenic research.ko_KR
dc.language.isoenko_KR
dc.publisherWileyko_KR
dc.subjectchickenko_KR
dc.subjectembryonic gonadsko_KR
dc.subjectgonadal primordial germ cell (gPGC)ko_KR
dc.subjectmass spectrometry (MS)ko_KR
dc.subjecttwo-dimensional gel electrophoresis (2-DE)ko_KR
dc.titleProteome analysis of chicken embryonic gonads: Identification of major proteins from cultured gonadal primordial germ cellsko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor한범구-
dc.contributor.AlternativeAuthor김진남-
dc.contributor.AlternativeAuthor신지혜-
dc.contributor.AlternativeAuthor김진규-
dc.contributor.AlternativeAuthor조도현-
dc.contributor.AlternativeAuthor김희발-
dc.contributor.AlternativeAuthor한재용-
dc.identifier.doi10.1002/mrd.20374-
Appears in Collections:
College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
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