S-Space College of Agriculture and Life Sciences (농업생명과학대학) Dept. of Food and Animal Biotechnology (식품·동물생명공학부) Journal Papers (저널논문_식품·동물생명공학부)
Gene expression profiling of chicken primordial germ cell ESTs
- Han, Jae Yong; Park, Tae Sub; Kim, Jin Nam; Kim, Mi A; Lim, Dajeong; Lim, Jeong Mook; Kim, Heebal
- Issue Date
- BioMed Central
- BMC Genomics, vol.30 no.7, 220-225
- Background: Germ cells are the only cell type that can penetrate from one generation to next
generation. At the early embryonic developmental stages, germ cells originally stem from
primordial germ cells, and finally differentiate into functional gametes, sperm in male or oocyte in
female, after sexual maturity. This study was conducted to investigate a large-scale expressed
sequence tag (EST) analysis in chicken PGCs and compare the expression of the PGC ESTs with
that of embryonic gonad.
Results: We constructed 10,851 ESTs from a chicken cDNA library of a collection of highly
separated embryonic PGCs. After chimeric and problematic sequences were filtered out using the
chicken genomic sequences, there were 5,093 resulting unique sequences consisting of 156 contigs
and 4,937 singlets. Pearson chi-square tests of gene ontology terms in the 2nd level between PGC
and embryonic gonad set showed no significance. However, digital gene expression profiling using
the Audic's test showed that there were 2 genes expressed significantly with higher number of
transcripts in PGCs compared with the embryonic gonads set. On the other hand, 17 genes in
embryonic gonads were up-regulated higher than those in the PGC set.
Conclusion: Our results in this study contribute to knowledge of mining novel transcripts and
genes involved in germline cell proliferation and differentiation at the early embryonic stages.
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