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Requirement of leukemia inhibitory factor for establishing and maintaining embryonic stem cells in mice
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Jae Hee | - |
dc.contributor.author | Lee, Eun Ju | - |
dc.contributor.author | Lee, Chae Hyun | - |
dc.contributor.author | Park, Jun Hong | - |
dc.contributor.author | Han, Jae Yong | - |
dc.contributor.author | Lim, Jeong Mook | - |
dc.date.accessioned | 2017-01-31T01:07:34Z | - |
dc.date.available | 2017-01-31T01:07:34Z | - |
dc.date.issued | 2009 | - |
dc.identifier.citation | Fertility and Sterility, vol.92 no.3, pp. 1133-1140 | ko_KR |
dc.identifier.issn | 0015-0282 | - |
dc.identifier.uri | https://hdl.handle.net/10371/100277 | - |
dc.description.abstract | Objective: To evaluate the necessity of leukemia inhibitory factor (LIF) in establishing and self-renewing embryonic stem cells (ESCs).
Design: Prospective animal model study. Setting: Gamete and Stem Cell Biotechnology Laboratory, Seoul National University, Korea. Animal(s): F1 hybrid B6D2F1 mice. Intervention(s): Inner cell mass (ICM) cells of blastocysts were cultured or commercially available ESCs were maintained in LIF-free or LIF-containing medium on mouse embryonic fibroblast (MEF) feeder. Main Outcome Measure(s): Cell morphology, LIF concentration, and mRNA expression. Result(s): The MEFs themselves secreted 146.5-175.3 pg/mL LIF in LIF-free medium. The ICM cells formed ESC-like colonies on MEF feeder, and E14 and R1 ESCs were successfully maintained in LIF-free medium. Expression of the genes either mediating LIF function or regulating stemness was not altered significantly, and change in the growth of ESCs was not prominent in LIF-free medium. Neither mRNA expression of differentiation-related genes nor differentiation into embryoid body was changed in the ESCs. Conclusion(s): Addition of LIF to culture medium is not necessary for establishing ICM-derived ESC-like colonies in the presence of fibroblast monolayer, and established ESCs can be maintained in an LIF-free medium. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | Elsevier | ko_KR |
dc.subject | Mouse | ko_KR |
dc.subject | ESCs | ko_KR |
dc.subject | LIF | ko_KR |
dc.subject | culture system | ko_KR |
dc.subject | stemness | ko_KR |
dc.subject | differentiation | ko_KR |
dc.title | Requirement of leukemia inhibitory factor for establishing and maintaining embryonic stem cells in mice | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 이재희 | - |
dc.contributor.AlternativeAuthor | 이은주 | - |
dc.contributor.AlternativeAuthor | 이채현 | - |
dc.contributor.AlternativeAuthor | 박준홍 | - |
dc.contributor.AlternativeAuthor | 한재용 | - |
dc.contributor.AlternativeAuthor | 임정묵 | - |
dc.identifier.doi | 10.1016/j.fertnstert.2008.07.1733 | - |
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