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Identification of the Major Proteins Produced by Cultured Germline Stem Cells in Chicken

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Authors
Han, Beom Ku; Jung, Jin Gyoung; Nam, Jungrye; Moon, Jong Kook; Kim, Jin Nam; Lee, Sang In; Kim, Jin Kyoo; Kim, Heebal; Han, Jae Yong
Issue Date
2009
Publisher
American Society of Andrology
Citation
Journal of Andrology, vol.30 no.6, pp. 690-702
Keywords
Spermatogonial stem cell2-dimensional gel electrophoresismass spectrometry
Abstract
Although chicken spermatogonial stem cells (SCs) are important in spermatogenesis and transgenic research, little is known about these cells. Recently, our group constructed an in vitro culture system to establish germline stem cells (GSCs). To examine the mechanism of chicken spermatogonial SC development, we constructed a proteome map of GSCs from 4-week-old chicken testes. Soluble extracts of the GSCs were fractionated by 2-dimensional gel electrophoresis (pH 4–7). Several protein spots, including those that displayed significantly high levels, were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry and liquid chromatography–tandem mass spectrometry. Of the 82/250 GSC spots examined, 56 yielded mass spectra that matched avian proteins found in the on-line databases. All of the identified proteins were classified into functional groups. This type of proteome map is an important resource for research on spermatogenesis and transgenesis.
ISSN
0196-3635
Language
English
URI
http://hdl.handle.net/10371/100280
DOI
https://doi.org/10.2164/jandrol.108.007401
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
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