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MicroRNA-mediated posttranscriptional regulation is required for maintaining undifferentiated properties of blastoderm and primordial germ cells in chickens

Cited 55 time in Web of Science Cited 57 time in Scopus
Authors
Lee, Sang In; Lee, Bo Ram; Hwang, Young Sun; Lee, Hyung Chul; Rengaraj, Deivendran; Song, Gwonhwa; Park, Tae Sub; Han, Jae Yong
Issue Date
2011
Publisher
National Academy of Sciences
Citation
Proceedings of the National Academy of Sciences, vol.108 no.26, pp. 10426-10431
Keywords
avesgermlinesex differentiation
Abstract
MicroRNAs (miRNAs) play a critical role in determining the differentiation fate of pluripotent stem cells and germ cells in mammals. However, the mechanism(s) of miRNA-mediated posttranscriptional regulation with regard to lineage specification and differentiation in chick development require further investigation. Therefore, we conducted miRNA expression profiling to explore specific miRNA signatures in undifferentiated blastoderm and primordial germ cells (PGCs). We identified seven miRNAs that are highly expressed in blastoderm and 10 that are highly expressed in PGCs. In this study, miR-302a and miR-456 for blastoderm and miR-181a* for PGCs were analyzed further for their target transcripts and regulatory pathways. Both miR-302a and miR-456 bound directly to the sex-determining region Y box 11 transcript and could act as posttranscriptional coregulators to maintain the undifferentiated state of the chicken blastoderm through the suppression of somatic gene expression and differentiation. Moreover, miR-181a* showed a bifunctional role in PGCs by binding to two different transcripts. miR-181a* inhibited the somatic differentiation of PGCs by silencing homeobox A1 expression. Additionally, miR-181a* prevented PGCs from entering meiosis through the repression of the nuclear receptor subfamily 6, group A, member 1 transcript. Collectively, our data demonstrate that in chickens miRNAs intrinsically regulate the differentiation fate of blastoderms and PGCs and that the specific timing of germcell meiosis is controlled through miRNA expression.
ISSN
0027-8424
Language
English
URI
http://hdl.handle.net/10371/100306
DOI
https://doi.org/10.1073/pnas.1106141108
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
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