Browse

Discovery of Candidate Genes and Pathways Regulating Oviduct Development in Chickens

Cited 35 time in Web of Science Cited 34 time in Scopus
Authors
Song, Gwonhwa; Seo, Hee Won; Choi, Jin Won; Rengaraj, Deivendran; Kim, Tae Min; Lee, Bo Ram; Kim, Young Min; Yun, Tae Won; Jeong, Jae-Wook; Han, Jae Yong
Issue Date
2011
Publisher
Society for the Study of Reproduction
Citation
Biology of Reproduction, vol.85 no.2, pp. 306-314
Keywords
chickendiethylstilbestrolestradiol/estradiol receptorestrogenfemale reproductive tractmicroarray analysisoviductuterus
Abstract
Estrogen is a key regulator in the development of the female reproductive system. It also stimulates oviduct development in immature chicks. We identified candidate genes and pathways associated with the development of chicken oviducts. A pellet containing the synthetic estrogen analog diethylstilbestrol (DES) was implanted subcutaneously in 1-wk-old female chicks for 10 days. The pellet was removed from half the group for 10 days, and an additional dose was given for a further 10 days. Total RNA was extracted from the oviducts of DES-treated and untreated chicks and subjected to an Affymetrix chicken GeneChip analysis. We found differential expression of 2290 and 1745 transcripts from the oviducts that were treated with DES once and twice, respectively. We also found a twofold or greater change in the expression of 77 and 390 transcripts between the two control and DES-treated time points, respectively, while we found a change in the expression of 10 transcripts that were common to all groups. Analyses of real-time PCR and in situ hybridization of selected genes confirmed the validity of the gene expression patterns observed in the microarray analysis. In particular, CCRN4L, FAM26F, HAS2, NELF, and NTM were upregulated in the DES-treated chicken oviducts. High-throughput analysis revealed that the differentially expressed genes were related to tubular formation, epithelial differentiation, hormone interactions, nerve development, and tissue remodeling in the chicken oviduct. This study provides novel insights into candidate genes regulating oviduct development and differentiation via estrogen. The identified genes may serve as biomarkers of reproductive tract development in chicks.
ISSN
0006-3363
Language
English
URI
http://hdl.handle.net/10371/100308
DOI
https://doi.org/10.1095/biolreprod.110.089227
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse