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Production of egg yolk antibodies specific to house dust mite proteins
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Kyung Eun | - |
dc.contributor.author | Han, Beom Ku | - |
dc.contributor.author | Han, Jae Yong | - |
dc.contributor.author | Hong, Jung Yeon | - |
dc.contributor.author | Kim, Mi Na | - |
dc.contributor.author | Heo, Won Il | - |
dc.contributor.author | Sohn, Myung Hyun | - |
dc.contributor.author | Kim, Kyung Won | - |
dc.contributor.author | Kim, Kyu Earn | - |
dc.date.accessioned | 2017-02-02T02:14:00Z | - |
dc.date.available | 2017-02-02T02:14:00Z | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | Yonsei Medical Journal, vol.55 no.4, pp. 999-1004 | ko_KR |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.uri | https://hdl.handle.net/10371/100378 | - |
dc.description | This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/ licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. | ko_KR |
dc.description.abstract | Purpose
House dust mites (HDMs) are an important source of indoor allergens associated with asthma, rhinitis and atopic dermatitis. Chicken immunoglobulin (Ig) Y is known to be a good alternative to mice and rabbit antibody production. In this study, we produced IgYs specific to HDMs and investigated their IgE immunoreactivities. Materials and Methods Total IgYs were isolated from the yolks of White Leghorn hens immunized with either Dermatophagoides pteronyssinus or D. farinae protein extract. Control antibodies were separated from the yolks of immunized hens with phosphate buffered saline. IgYs specific to HDMs were analyzed using enzyme-linked immunosorbent assay and Western blotting analysis. Results The concentration of egg IgY specific to D. farinae in an immunized hen increased and the highest achieved was 661.3 ug/mg (per an egg) on day 47, compared with 760 ug/mg IgY specific to D. pteronyssinus on day 16. The D. pteronyssinus or D. farinae-specific IgY was detected by binding of each mite proteins, and their immunoreactivities were elevated dependent of the specific IgY concentration. Conclusion IgY specific to HDMs may be a promising antibody for immunological diagnosis as well as identification of possible resistance relating to HDM allergy. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | Yonsei University College of Medicine | ko_KR |
dc.subject | House dust mite | ko_KR |
dc.subject | immunoglobulin Y | ko_KR |
dc.subject | D. pteronyssinus | ko_KR |
dc.subject | D. farinae | ko_KR |
dc.title | Production of egg yolk antibodies specific to house dust mite proteins | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 이경은 | - |
dc.contributor.AlternativeAuthor | 한범구 | - |
dc.contributor.AlternativeAuthor | 한재용 | - |
dc.contributor.AlternativeAuthor | 홍정연 | - |
dc.contributor.AlternativeAuthor | 김미나 | - |
dc.contributor.AlternativeAuthor | 허원일 | - |
dc.contributor.AlternativeAuthor | 손명현 | - |
dc.contributor.AlternativeAuthor | 김경원 | - |
dc.contributor.AlternativeAuthor | 김규언 | - |
dc.identifier.doi | 10.3349/ymj.2014.55.4.999 | - |
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