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Tumor evolution and intratumor heterogeneity of an epithelial ovarian cancer investigated using next-generation sequencing
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Jung-Yun | - |
dc.contributor.author | Yoon, Jung-Ki | - |
dc.contributor.author | Kim, Boyun | - |
dc.contributor.author | Kim, Soochi | - |
dc.contributor.author | Kim, Min A | - |
dc.contributor.author | Lim, Hyeonseob | - |
dc.contributor.author | Bang, Duhee | - |
dc.contributor.author | Song, Yong-Sang | - |
dc.date.accessioned | 2017-02-08T00:55:39Z | - |
dc.date.available | 2017-02-08T00:55:39Z | - |
dc.date.issued | 2015-02-26 | - |
dc.identifier.citation | BMC Cancer, 15(1):85 | ko_KR |
dc.identifier.uri | https://hdl.handle.net/10371/100516 | - |
dc.description | This is an Open Access article distributed under the terms of the Creative
Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. | ko_KR |
dc.description.abstract | Abstract
Background The extent to which metastatic tumors further evolve by accumulating additional mutations is unclear and has yet to be addressed extensively using next-generation sequencing of high-grade serous ovarian cancer. Methods Eleven spatially separated tumor samples from the primary tumor and associated metastatic sites and two normal samples were obtained from a Stage IIIC ovarian cancer patient during cytoreductive surgery prior to chemotherapy. Whole exome sequencing and copy number analysis were performed. Omental exomes were sequenced with a high depth of coverage to thoroughly explore the variants in metastatic lesions. Somatic mutations were further validated by ultra-deep targeted sequencing to sort out false positives and false negatives. Based on the somatic mutations and copy number variation profiles, a phylogenetic tree was generated to explore the evolutionary relationship among tumor samples. Results Only 6% of the somatic mutations were present in every sample of a given case with TP53 as the only known mutant gene consistently present in all samples. Two non-spatial clusters of primary tumors (cluster P1 and P2), and a cluster of metastatic regions (cluster M) were identified. The patterns of mutations indicate that cluster P1 and P2 diverged in the early phase of tumorigenesis, and that metastatic cluster M originated from the common ancestral clone of cluster P1 with few somatic mutations and copy number variations. Conclusions Although a high level of intratumor heterogeneity was evident in high-grade serous ovarian cancer, our results suggest that transcoelomic metastasis arises with little accumulation of somatic mutations and copy number alterations in this patient. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | BioMed Central | ko_KR |
dc.subject | High grade serous ovarian cancer | ko_KR |
dc.subject | Whole exome sequencing | ko_KR |
dc.subject | Intratumor heterogeneity | ko_KR |
dc.subject | Peritoneal seeding | ko_KR |
dc.subject | Transcoelomic metastasis | ko_KR |
dc.title | Tumor evolution and intratumor heterogeneity of an epithelial ovarian cancer investigated using next-generation sequencing | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 이정윤 | - |
dc.contributor.AlternativeAuthor | 윤정기 | - |
dc.contributor.AlternativeAuthor | 김보연 | - |
dc.contributor.AlternativeAuthor | 김수지 | - |
dc.contributor.AlternativeAuthor | 김민아 | - |
dc.contributor.AlternativeAuthor | 임현섭 | - |
dc.contributor.AlternativeAuthor | 방두희 | - |
dc.contributor.AlternativeAuthor | 송용상 | - |
dc.identifier.doi | 10.1186/s12885-015-1077-4 | - |
dc.language.rfc3066 | en | - |
dc.rights.holder | Lee et al.; licensee BioMed Central. | - |
dc.date.updated | 2017-01-06T10:11:22Z | - |
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