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Generation of protective immunity against Orientia tsutsugamushi infection by immunization with a zinc oxide nanoparticle combined with ScaA antigen

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Authors
Ha, Na-Young; Shin, Hyun Mu; Sharma, Prashant; Cho, Hyun Ah; Min, Chan-Ki; Kim, Hong-il; Yen, Nguyen Thi Hai; Kang, Jae-Seung; Kim, Ik-Sang; Choi, Myung-Sik; Kim, Young Keun; Cho, Nam-Hyuk
Issue Date
2016-11-26
Publisher
BioMed Central
Citation
Journal of Nanobiotechnology, 14(1):76
Keywords
Zinc oxide nanoparticleZnO binding peptideScrub typhusVaccine
Description
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License
(http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium,
provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license,
and indicate if changes were made.
Abstract
Abstract

Background
Zinc oxide nanoparticle (ZNP) has been applied in various biomedical fields. Here, we investigated the usage of ZNP as an antigen carrier for vaccine development by combining a high affinity peptide to ZNP.


Results
A novel zinc oxide-binding peptide (ZBP), FPYPGGDA, with high affinity to ZNP (K

a
 = 2.26 × 106 M−1) was isolated from a random peptide library and fused with a bacterial antigen, ScaA of Orientia tsutsugamushi, the causative agent of scrub typhus. The ZNP/ZBP-ScaA complex was efficiently phagocytosed by a dendritic cell line, DC2.4, in vitro and significantly enhanced anti-ScaA antibody responses in vivo compared to control groups. In addition, immunization with the ZNP/ZBP-ScaA complex promoted the generation of IFN-γ-secreting T cells in an antigen-dependent manner. Finally, we observed that ZNP/ZBP-ScaA immunization provided protective immunity against lethal challenge of O. tsutsugamushi, indicating that ZNP can be used as a potent adjuvant when complexed with ZBP-conjugated antigen.


Conclusions
ZNPs possess good adjuvant potential as a vaccine carrier when combined with an antigen having a high affinity to ZNP. When complexed with ZBP-ScaA antigen, ZNPs could induce strong antibody responses as well as protective immunity against lethal challenges of O. tsutsugamushi. Therefore, application of ZNPs combined with a specific soluble antigen could be a promising strategy as a novel vaccine carrier system.
Language
English
URI
http://hdl.handle.net/10371/100684
DOI
https://doi.org/10.1186/s12951-016-0229-2
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College of Medicine/School of Medicine (의과대학/대학원)Microbiology (미생물학전공)Journal Papers (저널논문_미생물학전공)
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