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Nuclear factor-kappaB activation correlates with better prognosis and Akt activation in human gastric cancer
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Byung Lan | - |
dc.contributor.author | Lee, Hye Seung | - |
dc.contributor.author | Jung, Jieun | - |
dc.contributor.author | Cho, Sung Jin | - |
dc.contributor.author | Chung, Hee-Yong | - |
dc.contributor.author | Kim, Woo Ho | - |
dc.contributor.author | Jin, Young-Woo | - |
dc.contributor.author | Kim, Chong Soon | - |
dc.contributor.author | Nam, Seon Young | - |
dc.date.accessioned | 2009-10-08T22:34:00Z | - |
dc.date.available | 2009-10-08T22:34:00Z | - |
dc.date.issued | 2005-04-01 | - |
dc.identifier.citation | Clin Cancer Res 2005;11(7):2518-25. | en |
dc.identifier.issn | 1078-0432 (Print) | - |
dc.identifier.uri | http://clincancerres.aacrjournals.org/content/11/7/2518.full | - |
dc.identifier.uri | https://hdl.handle.net/10371/10348 | - |
dc.description.abstract | PURPOSE: Because the biological significance of constitutive nuclear factor-kappaB (NF-kappaB) activation in human gastric cancer is unclear, we undertook this study to clarify the regulatory mechanism of NF-kappaB activation and its clinical significance. EXPERIMENTAL DESIGN: Immunohistochemistry for NF-kappaB/RelA was done on 290 human gastric carcinoma specimens placed on tissue array slides. The correlations between NF-kappaB activation and clinicopathologic features, prognosis, Akt activation, tumor suppressor gene expression, or Bcl-2 expression were analyzed. We also did luciferase reporter assay, Western blot analysis, and reverse transcription-PCR using the SNU-216 human gastric cancer cell line transduced with retroviral vectors containing constitutively active Akt or the NF-kappaB repressor mutant of IkappaBalpha. RESULTS: Nuclear expression of RelA was found in 18% of the gastric carcinomas and was higher in early-stage pathologic tumor-node-metastasis (P = 0.019). A negative correlation was observed between NF-kappaB activation and lymphatic invasion (P = 0.034) and a positive correlation between NF-kappaB activation and overall survival rate of gastric cancer patients (P = 0.0228). In addition, NF-kappaB activation was positively correlated with pAkt (P = 0.047), p16 (P = 0.004), adenomatous polyposis coli (P < 0.001), Smad4 (P = 0.002), and kangai 1 (P < 0.001) expression. An in vitro study showed that NF-kappaB activity in gastric cancer cells is controlled by and controls Akt. CONCLUSIONS: NF-kappaB activation was frequently observed in early-stage gastric carcinoma and was significantly correlated with better prognosis and Akt activation. These findings suggest that NF-kappaB activation is a valuable prognostic variable in gastric carcinoma. | en |
dc.description.sponsorship | Korea Research Foundation grant KRF-2003-003-E00163 and
BK21Project forMedicine, Dentistry, and Pharmacy (J. Jung and S. Cho). | en |
dc.language.iso | en | - |
dc.publisher | American Association for Cancer Research | en |
dc.subject | Adolescent | en |
dc.subject | Adult | en |
dc.subject | Aged | en |
dc.subject | Aged, 80 and over | en |
dc.subject | Androstadienes/pharmacology | en |
dc.subject | Blotting, Western | en |
dc.subject | Cell Line, Tumor | en |
dc.subject | Child | en |
dc.subject | Child, Preschool | en |
dc.subject | Chromones/pharmacology | en |
dc.subject | Enzyme Activation | en |
dc.subject | Female | en |
dc.subject | Gene Expression Regulation, Neoplastic/drug effects | en |
dc.subject | Genetic Vectors/genetics | en |
dc.subject | Humans | en |
dc.subject | Immunohistochemistry | en |
dc.subject | Infant | en |
dc.subject | Infant, Newborn | en |
dc.subject | Luciferases/genetics/metabolism | en |
dc.subject | Male | en |
dc.subject | Middle Aged | en |
dc.subject | Morpholines/pharmacology | en |
dc.subject | NF-kappa B/analysis/genetics/*metabolism | en |
dc.subject | Neoplasm Staging | en |
dc.subject | Prognosis | en |
dc.subject | Protein-Serine-Threonine Kinases/genetics/*metabolism | en |
dc.subject | Proto-Oncogene Proteins/genetics/*metabolism | en |
dc.subject | Proto-Oncogene Proteins c-akt | en |
dc.subject | Proto-Oncogene Proteins c-bcl-2/analysis | en |
dc.subject | RNA, Messenger/genetics/metabolism | en |
dc.subject | Recombinant Fusion Proteins/genetics/metabolism | en |
dc.subject | Retroviridae/genetics | en |
dc.subject | Reverse Transcriptase Polymerase Chain Reaction | en |
dc.subject | Stomach Neoplasms/genetics/metabolism/*pathology | en |
dc.subject | Survival Analysis | en |
dc.subject | Tissue Array Analysis | en |
dc.subject | Transcription Factor RelA | en |
dc.subject | Transfection | en |
dc.subject | Tumor Suppressor Proteins/analysis | en |
dc.title | Nuclear factor-kappaB activation correlates with better prognosis and Akt activation in human gastric cancer | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 이병란 | - |
dc.contributor.AlternativeAuthor | 이혜승 | - |
dc.contributor.AlternativeAuthor | 조성진 | - |
dc.contributor.AlternativeAuthor | 정희용 | - |
dc.contributor.AlternativeAuthor | 김우호 | - |
dc.contributor.AlternativeAuthor | 진영우 | - |
dc.contributor.AlternativeAuthor | 김종순 | - |
dc.contributor.AlternativeAuthor | 남선영 | - |
dc.contributor.AlternativeAuthor | 정지은 | - |
dc.identifier.doi | 10.1158/1078-0432.CCR-04-1282 | - |
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