Enhanced bone augmentation by controlled release of recombinant human bone morphogenic protein-2 from bioabsorbable membranes

Abstract

Background: The present study was undertaken to determine the effect of recombinant human bone morphogenetic protein- (rhBMP-2)-loaded biodegradable membranes on bone augmentation in a rabbit calvarial model.

Methods: Five µg of rhBMP-2 was loaded into a stiff hemispherical dome membrane made of poly(L-lactide) and tricalcium phosphate (PLLA/TCP). The release kinetics of rhBMP-2 from the membrane were determined in vitro using a human BMP-2 immunoassay. Twelve rhBMP-2-loaded dome membranes (test group) and 12 control dome membranes (control group) were placed on the partial-thickness calvarial defects of 24 rabbits. The animals were sacrificed at 4 and 8 weeks, and undecalcified ground sections were prepared. Newly formed bone area and height were measured histomorphometrically and calculated by percentage ratio to the total submembranous space area and height below the dome.