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Fetal gender determination and BclI polymorphism using nucleated erythrocytes in maternal blood

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Authors
Choe, Jin; Hwang, Doyeong; Kim, Ki-Chul; Choi, Young-Min
Issue Date
2005
Publisher
Histochemical Society
Citation
J Histochem Cytochem 53:323-327
Keywords
nucleated erythrocytefetal genderhemophilia Amicromanipulationprimer extensionpolymerase chain reaction
Abstract
This study demonstrated determination of fetal gender from nucleated red blood cells (NRBCs) in maternal blood and attempted to apply prenatal diagnosis of hemophilia A using BclI DNA polymorphism. Venous blood was drawn from 20 pregnant women, and NRBCs were recovered by magnetic activated cell sorting and anti-GPA (glycophorin A) immunostaining. After microdissector isolation of the NRBCs, primer extension preamplification (PEP) and nested PCR of the amelogenin gene were performed to determine fetal gender. We also performed PEP and nested PCR of BclI polymorphism to verify the validity of prenatal diagnosis of hemophilia A. DNA amplification was achieved in 107 cells (51.9%) and fetal gender determined with 65.0% accuracy. Unfortunately, we could not verify the validity within the scope of this study. However, in a larger number of cases that are informative in BclI polymorphism, we will be able to identify patients affected by hemophilia A using fetal NRBCs in maternal blood.
ISSN
0022-1554 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15750011

http://hdl.handle.net/10371/11056
DOI
https://doi.org/10.1369/jhc.4A6405.2005
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College of Medicine/School of Medicine (의과대학/대학원)Obstetrics & Gynecology (산부인과전공)Journal Papers (저널논문_산부인과학전공)
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