S-Space College of Medicine/School of Medicine (의과대학/대학원) Dermatology (피부과학전공) Journal Papers (저널논문_피부과학전공)
Topical application of 17beta-estradiol increases extracellular matrix protein synthesis by stimulating TGF-Beta signaling in aged human skin in vivo
- Son, Eui Dong; Lee, Jin Young; Lee, Serah; Kim, Mi Sun; Lee, Byeong Gon; Chang, Ih Seoup; Chung, Jin Ho
- Issue Date
- Nature Publishing Group
- J Invest Dermatol. 2005 Jun;124(6):1149-61.
- Administration, Topical; Adult; Aged; Aged, 80 and over; Antibodies/pharmacology; Cell Proliferation/drug effects; Collagen Type I/antagonists & inhibitors/genetics/metabolism; DNA-Binding Proteins/genetics/metabolism; Estradiol/*administration & dosage/pharmacology; Extracellular Matrix Proteins/*biosynthesis; Female; Humans; Keratinocytes/cytology; Male; Matrix Metalloproteinase 1/metabolism; Microfilament Proteins/genetics/metabolism; Middle Aged; Protein-Serine-Threonine Kinases; RNA, Messenger/metabolism; Receptors, Transforming Growth Factor beta/metabolism; Signal Transduction/*physiology; Skin Aging/*physiology; Smad3 Protein; Smad7 Protein; Trans-Activators/genetics/metabolism; Transforming Growth Factor beta/genetics/immunology/*metabolism; Transforming Growth Factor beta1; Tropoelastin/genetics/metabolism
- To investigate the effects of topically applied 17beta-estradiol on the expression of extracellular matrix proteins in aged human skin, 17beta-estradiol (0.01%) and its vehicle (70% propylene glycol, 30% ethanol) were applied to aged (68-82 y, eight females and five males) human buttock skin under occlusion for 2 wk (three times per week). Topical 17beta-estradiol was found to increase the expression of type 1 procollagen mRNA and protein significantly in human aged skin in vivo. In addition, metalloproteinase (MMP-1 protein levels were reduced by topical 17beta-estradiol. The expressions of TGF-beta1, TGF-beta type II receptor, and Sma and Mad related (Smad)3 were increased by topical 17 beta-estradiol in aged human skin, and TGF-beta1 neutralizing antibody inhibited 17beta-estradiol-induced procollagen synthesis in cultured fibroblasts. We also found that the expressions of tropoelastin and fibrillin-1 mRNA and protein, and elastic fibers in aged skin were also increased by topical 17beta-estradiol. Topical 17beta-estradiol also increased keratinocyte proliferation and the epidermal thickness in aged human skin. We also observed the same effects of topical 17beta-estradiol in young skin. In conclusion, our results suggest that topical 17beta-estradiol treatment may improve the cutaneous function of aged human skin by improving the connective tissue and increasing epidermal thickness.
- 0022-202X (Print)
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