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Topical application of 17beta-estradiol increases extracellular matrix protein synthesis by stimulating TGF-Beta signaling in aged human skin in vivo
Cited 88 time in
Web of Science
Cited 98 time in Scopus
- Authors
- Issue Date
- 2005-06-16
- Publisher
- Nature Publishing Group
- Citation
- J Invest Dermatol. 2005 Jun;124(6):1149-61.
- Keywords
- Administration, Topical ; Adult ; Aged ; Aged, 80 and over ; Antibodies/pharmacology ; Cell Proliferation/drug effects ; Collagen Type I/antagonists & inhibitors/genetics/metabolism ; DNA-Binding Proteins/genetics/metabolism ; Estradiol/*administration & dosage/pharmacology ; Extracellular Matrix Proteins/*biosynthesis ; Female ; Humans ; Keratinocytes/cytology ; Male ; Matrix Metalloproteinase 1/metabolism ; Microfilament Proteins/genetics/metabolism ; Middle Aged ; Protein-Serine-Threonine Kinases ; RNA, Messenger/metabolism ; Receptors, Transforming Growth Factor beta/metabolism ; Signal Transduction/*physiology ; Skin Aging/*physiology ; Smad3 Protein ; Smad7 Protein ; Trans-Activators/genetics/metabolism ; Transforming Growth Factor beta/genetics/immunology/*metabolism ; Transforming Growth Factor beta1 ; Tropoelastin/genetics/metabolism
- Abstract
- To investigate the effects of topically applied 17beta-estradiol on the expression of extracellular matrix proteins in aged human skin, 17beta-estradiol (0.01%) and its vehicle (70% propylene glycol, 30% ethanol) were applied to aged (68-82 y, eight females and five males) human buttock skin under occlusion for 2 wk (three times per week). Topical 17beta-estradiol was found to increase the expression of type 1 procollagen mRNA and protein significantly in human aged skin in vivo. In addition, metalloproteinase (MMP-1 protein levels were reduced by topical 17beta-estradiol. The expressions of TGF-beta1, TGF-beta type II receptor, and Sma and Mad related (Smad)3 were increased by topical 17 beta-estradiol in aged human skin, and TGF-beta1 neutralizing antibody inhibited 17beta-estradiol-induced procollagen synthesis in cultured fibroblasts. We also found that the expressions of tropoelastin and fibrillin-1 mRNA and protein, and elastic fibers in aged skin were also increased by topical 17beta-estradiol. Topical 17beta-estradiol also increased keratinocyte proliferation and the epidermal thickness in aged human skin. We also observed the same effects of topical 17beta-estradiol in young skin. In conclusion, our results suggest that topical 17beta-estradiol treatment may improve the cutaneous function of aged human skin by improving the connective tissue and increasing epidermal thickness.
- ISSN
- 0022-202X (Print)
- Language
- English
- URI
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15955089
https://hdl.handle.net/10371/11561
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