Browse

Dexamethasone-conjugated low molecular weight polyethylenimine as a nucleus-targeting lipopolymer gene carrier

Cited 67 time in Web of Science Cited 59 time in Scopus
Authors
Bae, Yun Mi; Choi, Hye; Lee, Seungah; Kang, Seong Ho; Kim, Young Tae; Nam, Kihoon; Park, Jong Sang; Lee, Minhyung; Choi, Joon Sig
Issue Date
2007-09-14
Publisher
American Chemical Society
Citation
Bioconjugate Chem. 2007 Nov-Dec;18(6):2029-36. Epub 2007 Sep 13.
Keywords
Cell LineCell Nucleus/drug effects/geneticsCell Survival/drug effectsDNA/chemistry/geneticsDexamethasone/*chemistry/toxicityElectrophoresis, Agar GelHumansLipids/*chemistryMagnetic Resonance SpectroscopyMolecular StructureMolecular WeightPlasmids/chemistry/geneticsPolyethyleneimine/*chemistryTransfection
Abstract
Dexamethasone, a glucocorticoid steroid, can dilate the nuclear pore complexes and translocate into the nucleus when it is bound to its glucocorticoid receptor, suggesting that the transport of DNA into the nucleus may be facilitated by the reagent. In this research, dexamethasone was conjugated to low molecular weight polyethylenimine (2 kDa) for efficient translocation of the polymer/DNA complex into the nucleus. Polyethylenimine (PEI)-dexamethasone (PEI-Dexa) was synthesized by one-step reaction using the Traut's reagent. In gel retardation assay, the PEI-Dexa/DNA complex was completely retarded at or above 0.3/1 weight ratio (polymer/DNA). The average size distributions and zeta-potential values of the complexes were measured at various weight ratios. In vitro transfection assay showed that the PEI-Dexa/DNA complex had higher gene delivery efficiency compared to PEI 2kDa/DNA complex. The localization of PEI-Dexa/plasmid DNA complexes in the nucleus was confirmed by using total internal reflection fluorescence and Nomarski differential interference contrast microscope as well as confocal microscope. Therefore, with efficient nuclear translocation and low cytotoxicity, PEI-Dexa may be useful for nonviral gene therapy.
ISSN
1043-1802 (Print)
Language
English
URI
http://hdl.handle.net/10371/11587
DOI
https://doi.org/10.1021/bc070012a
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Thoracic Surgery (흉부외과학전공)Journal Papers (저널논문_흉부외과학전공)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse