S-Space College of Medicine/School of Medicine (의과대학/대학원) Orthopedic Surgery (정형외과학전공) Journal Papers (저널논문_정형외과학전공)
Detection and identification of Mycobacterium tuberculosis in joint biopsy specimens by rpoB PCR cloning and sequencing
- Yun, Yeo-Jun; Lee, Keun-Hwa; Haihua, Lin; Ryu, Yoon-Jong; Kim, Bum-Joon; Lee, Young-Ho; Baek, Goo-Hyun; Kim, Hee-Joong; Chung, Moon-Sang; Lee, Myung-Chul; Lee, Sang-Hoon; Choi, In-Ho; Cho, Tae-Jun; Chang, Bong-Soon; Kook, Yoon-Hoh
- Issue Date
- American Society for Microbiology
- J Clin Microbiol. 2005 Jan;43(1):174-8.
- Antitubercular Agents/pharmacology; Biopsy; Cloning, Molecular; DNA-Directed RNA Polymerases/*genetics; Humans; Joints/*microbiology; Mycobacterium tuberculosis/*classification/drug; effects/genetics/*isolation & purification; Plasmids; Polymerase Chain Reaction/*methods; Rifampin/pharmacology; Sequence Analysis, DNA; Tuberculosis, Osteoarticular/*microbiology
- Osteoarticular tuberculosis (OAT) is an extrapulmonary tuberculosis and accounts for 1 to 3% of all tuberculosis cases. We used an rpoB PCR-plasmid TA cloning-sequencing method to detect and identify tubercle bacilli in surgical specimens from patients suspected of having OAT. By comparing the similarities of the rpoB sequences determined with those in GenBank, Mycobacterium tuberculosis was detected in 23 of 43 samples. Three of the 23 positive samples had mutations at codon 531, which are commonly observed in rifampin-resistant M. tuberculosis strains. Our results suggest that the rpoB PCR-TA cloning-sequencing method developed, which detects M. tuberculosis and which simultaneously determines its rifampin susceptibility, can also be used efficiently for the diagnosis of OAT.
- 0095-1137 (Print)
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