SHERP

Association of the invasion ability of Porphyromonas gingivalis with the severity of periodontitis

Cited 17 time in webofscience Cited 0 time in scopus
Authors
Baek, Keum Jin; Ji, Suk; Kim, Yong Chul; Choi, Youngnim
Issue Date
2015-01-23
Publisher
BK21플러스치의학생명과학단&치학연구소
Citation
Virulence, Vol.6 No.3, pp. 274-281
Keywords
Association of the invasion ability of Porphyromonas gingivalis with the severity of periodontitis의약학cytokine proteolytic activityinvasion abilityPorphyromonas gingivalisperiodontitisseverity
Abstract
Porphyromonas gingivalis is one of the well-characterized periodontal pathogens involved in periodontitis. The invasive and proteolytic activities of P. gingivalis clinical isolates have been shown to be associated with heterogenic virulence, as determined in a mouse abscess model. The aims of the present study were to identify a P. gingivalis strain with a low virulence among clinical isolates, based on its invasive ability and cytokine proteolytic activities, and to explore the preferential degradation of a certain cytokine by P. gingivalis. P. gingivalis ATCC 33277, W50, and 10 clinical isolates were used. After incubating bacteria with IL-4, IL-6, IL-10, IL-17A, TNFα, IFNγ, and IL-1α, the amounts of remaining cytokines were determined by ELISA. Invasion ability was measured by a flow cytometric invasion assay. There was inter-strain variability both in the cytokine proteolytic activities and invasion ability. In addition, differential degradation of cytokines by P. gingivalis was observed: while IFNγ and IL-17A were almost completely degraded, inflammatory cytokines TNFα and IL-1α were less susceptible to degradation. Interestingly, the invasion index, but not cytokine proteolytic activities, of P. gingivalis had strong positive correlations with clinical parameters of subjects who harbored the isolates. Therefore, the invasive ability of P. gingivalis is an important virulence factor, and the bacterial invasion step may be a good target for new therapeutics of periodontitis.
ISSN
2150-5594
URI
http://hdl.handle.net/10371/116869
DOI
https://doi.org/10.1080/21505594.2014.1000764
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse