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Imaging Single-mRNA Localization and Translation in Live Neurons

Cited 11 time in Web of Science Cited 9 time in Scopus
Authors
Lee, Byung Hun; Bae, Seong-Woo; Shim, Jaeyoun Jay; Park, Sung Young; Park, Hye Yoon
Issue Date
2016-12
Publisher
Korean Society for Molecular and Cellular Biology
Citation
Molecules and Cells, Vol.39 No.12, pp. 841-846
Keywords
Imaging Single-mRNA Localization and Translation in Live Neurons자연과학live-cell imaginglocal translationMS2-GFPRNA localizationsingle-molecule imaging
Abstract
Local protein synthesis mediates precise spatio-temporal regulation of gene expression for neuronal functions such as long-term plasticity, axon guidance and regeneration. To reveal the underlying mechanisms of local translation, it is crucial to understand mRNA transport, localization and translation in live neurons. Among various techniques for mRNA analysis, fluorescence microscopy has been widely used as the most direct method to study localization of mRNA. Live-cell imaging of single RNA molecules is particularly advantageous to dissect the highly heterogeneous and dynamic nature of messenger ribonucleoprotein (mRNP) complexes in neurons. Here, we review recent advances in the study of mRNA localization and translation in live neurons using novel techniques for single-RNA imaging.
ISSN
1016-8478
Language
English
URI
http://hdl.handle.net/10371/116885
DOI
https://doi.org/10.14348/molcells.2016.0277
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College of Natural Sciences (자연과학대학)Dept. of Physics and Astronomy (물리·천문학부)Physics (물리학전공)Journal Papers (저널논문_물리학전공)
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