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Time-averaged simulated microgravity (taSMG) inhibits proliferation of lymphoma cells, L-540 and HDLM-2, using a 3D clinostat

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Authors
Kim, Yoon Jae; Jeong, Ae Jin; Kim, Myungjoon; Lee, Chiwon; Ye, Sang-Kyu; Kim, Sungwan
Issue Date
2017-04-20
Publisher
BimMed Central
Citation
BioMedical Engineering OnLine, 16(1):48
Keywords
MicrogravityLymphoma3D clinostatDermal fbroblast
Abstract
Background
Gravity is omnipresent on Earth; however, humans in space, such as astronauts at the International Space Station, experience microgravity. Long-term exposure to microgravity is considered to elicit physiological changes, such as muscle atrophy, in the human body. In addition, certain types of cancer cells demonstrate inhibited proliferation under condition of time-averaged simulated microgravity (taSMG). However, the response of human Hodgkin’s lymphoma cancer cells to reduced gravity, and the associated physiological changes in these cells, have not been elucidated.

Methods
In this study, the proliferation of human Hodgkin’s lymphoma cancer cells (L-540 and HDLM-2) under taSMG condition (<10−3 G, 1 G is defined as 9.8 m/s2) was studied using a 3D clinostat. Normal human dermal fibroblast (HDF) was proliferated in the same condition as a control group. For the development of 3D clinostat, two motors were used to actuate the frames. Electrical wires for power supply and communication were connected via slip ring. For symmetrical path of gravitational vector, optimal angular velocities of the motors were found using simulation results. Under the condition of taSMG implemented by the 3D clinostat, proliferation of the cells was observed for 3 days.

Results
The results indicated that proliferation of these cancer cells was significantly (p < 0.0005) inhibited under taSMG, whereas proliferation of normal HDF cells was not affected.

Conclusions
Findings in this study could be significantly valuable in developing novel strategies for selective killing of cancer cells such as lymphoma.
Language
English
URI
https://doi.org/10.1186/s12938-017-0337-8

http://hdl.handle.net/10371/117014
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College of Medicine/School of Medicine (의과대학/대학원)Pharmacology (약리학전공)Journal Papers (저널논문_약리학전공)
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