Tissue-engineered growth of bone by marrow cell transplantation using porous calcium metaphosphate matrices

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Lee, Yong-Moo; Seol, Yang-Jo; Lim, Yun-Tak; Kim, Sukyoung; Han, Soo-Boo; Rhyu, In-Chul; Baek, Seung-Hak; Heo, Seong-Joo; Choi, Jin-Young; Klokkevold, Perry R.; Chung, Chong-Pyoung
Issue Date
John Wiley & Sons
J Biomed Mater Res 2000;54:216-223
porous calcium metaphosphate (CMP) matrixtissue engineeringmarrow-derived mesenchymal cellstransplantationbone regeneration
In this study we investigated not only osteoblastic cell proliferation and differentiation on the surface of calcium metaphosphate (CMP) matrices in vitro but also bone formation by ectopic implantation of these cell-matrix constructs in athymic mice in vivo. Interconnected porous CMP matrices with pores 200 m in size were prepared to use as scaffolds for rat-marrow stromal-cell attachment. Cell-matrix constructs were cultured in vitro, and cell proliferation and ALPase activities were monitored for 56 days. In addition to their being cultured in vitro, cell-matrix constructs were implanted into subcutaneous sites of athymic mice. In vitro these porous CMP matrices supported the proliferation of osteoblastic cells as well as their differentiation, as indicated by high ALPase activity. In vivo the transplanted marrow cells gave rise to bone tissues in the pores of the CMP matrices. A small amount of woven bone formation was detected first at 4 weeks; osteogenesis progressed vigorously with time, and thick lamellar bones that had been remodeled were observed at 12 weeks. These findings demonstrate the potential for using a porous CMP matrix as a biodegradable scaffold ex vivo along with attached marrow-derived mesenchymal cells for transplantation into a site for bone regeneration in vivo.
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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