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Tissue-engineered growth of bone by marrow cell transplantation using porous calcium metaphosphate matrices
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Yong-Moo | - |
dc.contributor.author | Seol, Yang-Jo | - |
dc.contributor.author | Lim, Yun-Tak | - |
dc.contributor.author | Kim, Sukyoung | - |
dc.contributor.author | Han, Soo-Boo | - |
dc.contributor.author | Rhyu, In-Chul | - |
dc.contributor.author | Baek, Seung-Hak | - |
dc.contributor.author | Heo, Seong-Joo | - |
dc.contributor.author | Choi, Jin-Young | - |
dc.contributor.author | Klokkevold, Perry R. | - |
dc.contributor.author | Chung, Chong-Pyoung | - |
dc.date.accessioned | 2009-11-11T05:41:17Z | - |
dc.date.available | 2009-11-11T05:41:17Z | - |
dc.date.issued | 2000 | - |
dc.identifier.citation | J Biomed Mater Res 2000;54:216-223 | en |
dc.identifier.issn | 1549-3296 | - |
dc.identifier.uri | https://hdl.handle.net/10371/11900 | - |
dc.description.abstract | In this study we investigated not only osteoblastic cell proliferation and differentiation on the surface of calcium metaphosphate (CMP) matrices in vitro but also bone formation by ectopic implantation of these cell-matrix constructs in athymic mice in vivo. Interconnected porous CMP matrices with pores 200 m in size were prepared to use as scaffolds for rat-marrow stromal-cell attachment. Cell-matrix constructs were cultured in vitro, and cell proliferation and ALPase activities were monitored for 56 days. In addition to their being cultured in vitro, cell-matrix constructs were implanted into subcutaneous sites of athymic mice. In vitro these porous CMP matrices supported the proliferation of osteoblastic cells as well as their differentiation, as indicated by high ALPase activity. In vivo the transplanted marrow cells gave rise to bone tissues in the pores of the CMP matrices. A small amount of woven bone formation was detected first at 4 weeks; osteogenesis progressed vigorously with time, and thick lamellar bones that had been remodeled were observed at 12 weeks. These findings demonstrate the potential for using a porous CMP matrix as a biodegradable scaffold ex vivo along with attached marrow-derived mesenchymal cells for transplantation into a site for bone regeneration in vivo. | en |
dc.language.iso | en | en |
dc.publisher | John Wiley & Sons | en |
dc.subject | porous calcium metaphosphate (CMP) matrix | en |
dc.subject | tissue engineering | en |
dc.subject | marrow-derived mesenchymal cells | en |
dc.subject | transplantation | en |
dc.subject | bone regeneration | en |
dc.title | Tissue-engineered growth of bone by marrow cell transplantation using porous calcium metaphosphate matrices | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 이용무 | - |
dc.contributor.AlternativeAuthor | 설양조 | - |
dc.contributor.AlternativeAuthor | 임윤택 | - |
dc.contributor.AlternativeAuthor | 김석영 | - |
dc.contributor.AlternativeAuthor | 한수부 | - |
dc.contributor.AlternativeAuthor | 류인철 | - |
dc.contributor.AlternativeAuthor | 백승학 | - |
dc.contributor.AlternativeAuthor | 허성주 | - |
dc.contributor.AlternativeAuthor | 최진영 | - |
dc.contributor.AlternativeAuthor | 정종평 | - |
dc.identifier.doi | 10.1002/1097-4636(200102)54:2<216::AID-JBM8>3.0.CO;2-C | - |
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