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Studies on novel DNA signatures of telomerase-independent telomere maintenance in Caenorhabditis elegans
대안적 텔로미어 유지기작의 새로운 DNA 복제단위 연구

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Authors
김천아
Advisor
이준호
Major
자연과학대학 생명과학부
Issue Date
2016-02
Publisher
서울대학교 대학원
Keywords
C. elegansgeneticstelomeretelomere maintenance mechanismalternative lengthening of telomeres (ALT)template for ALT (TALT)animal model for telomerase-independent survivor
Description
학위논문 (박사)-- 서울대학교 대학원 : 생명과학부, 2016. 2. 이준호.
Abstract
Telomeres at linear chromosomal ends cannot be replicated by DNA polymerase. This is called the end replication problem. If this problem is not solved, chromosomes will gradually be shortened along cell divisions. In order to maintain chromosome integrity through generations, organisms have invented telomere maintenance mechanisms. A specialized reverse transcriptase, telomerase, has evolved in ancient eukaryotic cells. Interestingly, telomerase has been lost a few times during evolution. In this situation, telomerase-independent mechanisms were adopted. Among telomerase-independent mechanisms, alternative lengthening of telomeres (ALT) has been studied in cancer formation. However, many studies on ALT did not have organismal perspectives. Here, I establish a model for organismal telomere maintenance after telomerase loss. Using Caenorhabditis elegans telomerase mutant, I have generated telomerase-independent survivors. These survivors mobilized specific internal sequence blocks for telomere lengthening, which we named TALTs (Templates for ALT). These TALTs region consist of a block of genomic DNA flanked by telomere-like sequences. Telomerase-independent survivors trans-duplicate TALTs, which is already cis-duplicated to chromosome ends, across the telomeres of all chromosomes. The switch from the telomerase-mediated to the TALT-mediated telomere maintenance mechanism shows a snapshot of telomere evolution. In addition, the molecular event of the cis-duplication of TALTs suggests a novel mechanism for subtelomere formation
Language
English
URI
http://hdl.handle.net/10371/121440
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College of Natural Sciences (자연과학대학)Dept. of Biological Sciences (생명과학부)Theses (Ph.D. / Sc.D._생명과학부)
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