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Studies on novel DNA signatures of telomerase-independent telomere maintenance in Caenorhabditis elegans : 대안적 텔로미어 유지기작의 새로운 DNA 복제단위 연구
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 이준호 | - |
dc.contributor.author | 김천아 | - |
dc.date.accessioned | 2017-07-14T00:51:48Z | - |
dc.date.available | 2017-07-14T00:51:48Z | - |
dc.date.issued | 2016-02 | - |
dc.identifier.other | 000000132396 | - |
dc.identifier.uri | https://hdl.handle.net/10371/121440 | - |
dc.description | 학위논문 (박사)-- 서울대학교 대학원 : 생명과학부, 2016. 2. 이준호. | - |
dc.description.abstract | Telomeres at linear chromosomal ends cannot be replicated by DNA polymerase. This is called the end replication problem. If this problem is not solved, chromosomes will gradually be shortened along cell divisions. In order to maintain chromosome integrity through generations, organisms have invented telomere maintenance mechanisms. A specialized reverse transcriptase, telomerase, has evolved in ancient eukaryotic cells. Interestingly, telomerase has been lost a few times during evolution. In this situation, telomerase-independent mechanisms were adopted. Among telomerase-independent mechanisms, alternative lengthening of telomeres (ALT) has been studied in cancer formation. However, many studies on ALT did not have organismal perspectives. Here, I establish a model for organismal telomere maintenance after telomerase loss. Using Caenorhabditis elegans telomerase mutant, I have generated telomerase-independent survivors. These survivors mobilized specific internal sequence blocks for telomere lengthening, which we named TALTs (Templates for ALT). These TALTs region consist of a block of genomic DNA flanked by telomere-like sequences. Telomerase-independent survivors trans-duplicate TALTs, which is already cis-duplicated to chromosome ends, across the telomeres of all chromosomes. The switch from the telomerase-mediated to the TALT-mediated telomere maintenance mechanism shows a snapshot of telomere evolution. In addition, the molecular event of the cis-duplication of TALTs suggests a novel mechanism for subtelomere formation | - |
dc.description.tableofcontents | Introduction 1
1. Telomeres: protecting chromosomes ends 2 2. Telomerase and alternative lengthening of telomeres 3 3. Ancient mechanisms of telomere maintenance 6 4. Alternative means of lengthening telomeres in nature 7 5. Purposes of this study 8 Materials and Methods 12 Results 26 1. Isolation of telomerase-independent survivors of C. elegans 27 2. Telomere maintenance by TALT in survivors 30 3. ALT mechanism involves TALT duplication in cis and in trans 33 4. Inducer of TALT trans-duplication 36 Discussion 40 1. Unique structure of TALT sequences 41 2. DNA damage response: a possible mechanism of trans-duplication 43 3. Two types of ALT mechanisms in C. elegans 45 4. Cis-duplication of TALT in subtelomeres evolution 46 5. Remaining questions 49 References 125 Abstract in Korean 135 | - |
dc.format | application/pdf | - |
dc.format.extent | 7278845 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | C. elegans | - |
dc.subject | genetics | - |
dc.subject | telomere | - |
dc.subject | telomere maintenance mechanism | - |
dc.subject | alternative lengthening of telomeres (ALT) | - |
dc.subject | template for ALT (TALT) | - |
dc.subject | animal model for telomerase-independent survivor | - |
dc.subject.ddc | 570 | - |
dc.title | Studies on novel DNA signatures of telomerase-independent telomere maintenance in Caenorhabditis elegans | - |
dc.title.alternative | 대안적 텔로미어 유지기작의 새로운 DNA 복제단위 연구 | - |
dc.type | Thesis | - |
dc.description.degree | Doctor | - |
dc.citation.pages | 136 | - |
dc.contributor.affiliation | 자연과학대학 생명과학부 | - |
dc.date.awarded | 2016-02 | - |
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