SHERP

Changes in skin during distraction osteogenesis of the tibia in Sprague-Dawley rats: verification of epidermal proliferation by immunohistochemical methods

Cited 0 time in webofscience Cited 4 time in scopus
Authors
Chang, Hak; Kwon, Sung Tack; Chung, Chin Youb; Choi, In Ho; Ahn, Heu Tae; Cho, Kwang Hyun
Issue Date
2007
Publisher
Taylor & Francis
Citation
Scand J Plast Reconstr Surg Hand Surg 2007;41:97-102
Keywords
AnimalsAntimetabolites/metabolismBromodeoxyuridine/metabolismCell ProliferationExternal FixatorsImmunohistochemistryModels, Animal*Osteogenesis, DistractionRatsRats, Sprague-DawleySkin/*cytology/metabolism/ultrastructureTibia/surgery
Abstract
To investigate the effect of bone lengthening by distraction osteogenesis, a pair of external fixators was placed in the right tibias of 40 Sprague-Dawley rats. Two weeks (n=10) and 12 months (n=10) after 25% lengthening, skin flaps were removed and prepared for the immunohistochemical measurement of bromodeoxyuridine (BrdU) and staining with haematoxylin and eosin. The same procedures were done for the rest of 20 unlengthened tibias. At 2 weeks the number of cells labelled with BrdU, which indicate the extent of DNA synthesis, was 4.35 times (p<0.001) greater in the lengthened than the unlengthened skin. However, at 12 months, there was no difference. Haematoxylin and eosin staining showed no additional findings. Although the physical properties of the skin can accommodate a lengthened bone, our results suggest that distraction osteogenesis stimulates epidermal proliferation, which might lead to an increase in the amount of skin.
ISSN
0284-4311 (Print)
Language
English
URI
http://www.informaworld.com/smpp/content~db=all?content=10.1080/02844310701214438

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17486513

http://hdl.handle.net/10371/12212
DOI
https://doi.org/10.1080/02844310701214438
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Orthopedic Surgery (정형외과학전공)Journal Papers (저널논문_정형외과학전공)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse