S-Space College of Engineering/Engineering Practice School (공과대학/대학원) Dept. of Material Science and Engineering (재료공학부) Theses (Master's Degree_재료공학부)
Hydrogel microfluidic 3D culture of lung cancer cells mimicking cancer microenvironment
폐암 주변 미세환경을 모사한 하이드로젤에서 마이크로플루이딕 3차원적 폐암세포 배양
- Dayoung Yoon
- 공과대학 재료공학부
- Issue Date
- 서울대학교 대학원
- 학위논문 (석사)-- 서울대학교 대학원 : 재료공학부, 2016. 8. 안철희.
- In vitro, lung cancer has been studied in 2D, tissue culture plate by many cancer researchers. Recently, changes of study trend in microenvironment have moved 2D to 3D. Many papers have reported differences of results in 2D culture and 3D culture. The in vitro results in 3D culture were more similar to in vivo results than those in 2D. Thus, many researchers have studied cells using hydrogels. One of the hydrogels is collagen type Ⅰ that is a most common extracellular matrix (ECM). However, the composition of ECM in each tissue is different due to the different function. Lungs are organs in respiratory system, and those have elasticity for extension and contraction. The ECM in lungs has known that collagen and elastin are the major components. Our study focused on lung microenvironment, and using microfluidic devices we demonstrated lung cancer cells in hydrogels mimicking lung ECM which are composed of collagen type Ⅰ, collagen type Ⅲ, and elastin (CCE). These results have shown that differences of morphologies and bioactivities in RNA levels, protein levels, and enzyme levels are remarkable. Furthermore, our studies suggest that difference of hydrogel property could affect responses of lung cancer cells.