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Transcription factor gene fgzc248 is involved in DNA damage response in Fusarium graminearum : Fusarium graminearum의 전사조절인자 FgZC248의 기능연구
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- Authors
- Advisor
- 이인원 교수님
- Major
- 농업생명과학대학 농생명공학부
- Issue Date
- 2014-08
- Publisher
- 서울대학교 대학원
- Keywords
- DNA damage response ; Fusarium graminearum ; transcription factor ; hydroxyurea
- Description
- 학위논문 (석사)-- 서울대학교 대학원 : 농생명공학부, 2014. 8. 이인원.
- Abstract
- Fusarium graminearum is an important plant pathogen which causes Fusarium head blight in cereal crops. The maintenance of genome integrity plays important role in the survival or virulence of the plant pathogen since DNA acts as the carrier of genetic information. Transcription factors (TFs) orchestrate gene expression under the control of various cellular signaling pathways and therefore function as key mediators of cellular functions. To identify TFs related to DNA damage responses in F. graminearum, four different DNA damage agents were used to screen the previously generated F. graminearum transcription factor mutant library. Sixteen transcription factor gene deletion mutants showed altered sensitivity to at least one DNA damage agent. Among them, the fgzc248 deletion mutant was specifically sensitive to hydroxyurea than the wild-type strain. Moreover, deletion of fgzc248 resulted in defects in virulence and sexual development. Result of the alkaline comet assay demonstrated that disruption of fgzc248 function resulted in DNA damages in cells. Levels of transcript accumulation of DNA repair genes increased in the fgzc248 deletion mutant compared with wild-type. This study is the first characterizing a novel transcription factor FgZC248 involved in DNA damage response and will provide new insights in mechanisms underlying DNA damage responses in F. graminearum.
- Language
- English
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