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Antifungal efficacy of a synthetic human β-defensin-3-C15 against Candida albicans-infected dentin infection model

DC Field Value Language
dc.contributor.advisor금기연-
dc.contributor.author권이경-
dc.date.accessioned2017-07-19T08:19:59Z-
dc.date.available2017-07-19T08:19:59Z-
dc.date.issued2016-02-
dc.identifier.other000000133132-
dc.identifier.urihttp://hdl.handle.net/10371/130854-
dc.description학위논문 (석사)-- 서울대학교 치의학대학원 : 치의학대학원 치의학과, 2016. 2. 금기연.-
dc.description.abstractObjectives
The purposes of this ex vivo study were (1) to assess the anti-fungal efficacy of a synthetic human β-defensin-3-C15 (HBD3-C15, 15 amino acids in length) peptide against C. albicans biofilms and (2) to evaluate the drug potential of synthetic HBD3-C15 peptide as a substitute of calcium hydroxide drug in the fungi-infected root canal. In addition, (3) a recent issue regarding the ‘Human Root Dentin Block’ approach that proposed the usefulness as ex vivo intra-canal fungal infection model was also discussed.

Materials and methods
Six millimeter height of human dentin blocks (internal diameter: 0.5 mm) were infected with C. albicans for three weeks. Non-medicament gel (n = 8), satu-rated calcium hydroxide mixed with distilled water (n = 8), non-functional HBD3 peptide gel (n = 8), and synthetic HBD3-C15 peptide gel (n = 8), were tested. After one week of medicament, the debris of dentinal tubules at the depth of 200 and 400 µm were collected using Gates-Glidden drills from root canal lumen. Efficacy of medicaments was assessed by measuring Colony Forming Unit (CFU) of C. albicans after 72 hours of 37°C, 5% CO2 incuba-tion. Data were statistical analyzed with Kruskal-Wallis and Wilcoxon test us-ing SPSS version 23.

Results
The synthetic HBD3-C15 peptide group demonstrated significantly lower CFU values than the non-medicament gel group at both depths (p < 0.05). The syn-thetic HBD3-C15 peptide group also shows significantly lower CFU values than the non-functional HBD3 peptide group at both depths (p < 0.05). None-theless, there was no significant difference in the CFU values between syn-thetic HBD3-C15 and calcium hydroxide at both depths (p > 0.05).
There was no significant difference in the CFU values of the inner layer and outer dentinal layer for any group (p > 0.05).

Conclusions
The synthetic HBD3-C15 peptide has an anti-fungal efficacy against C. albicans biofilms. The synthetic HBD3-C15 peptide showed comparable anti-fungal activity to calcium hydroxide group in C. albicans-infected human root dentin model. The dentin block model effectively reproduced ex vivo situation to evaluate anti-fungal activity of intra-canal medicaments.

Keywords
Anti-fungal efficacy, human dentin block, C. albicans biofilms, synthetic hu-man β-defensin-3-C15 peptide, colony forming unit.

Student number
2012-22140
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dc.description.tableofcontentsⅠ. INTRODUCTION 3

Ⅱ. MATERIALS AND METHODS 7
1. Human root dentin block model 7
2. Infection of C. albicans biofilms into human dentin blocks 7
3. Assessment of anti-fungal activity 7
4. Statistical analysis 8

Ⅲ. RESULTS 9
1. Confirmation of biofilm formation and dentinal tubule infection 9
2. Measurement of Colony Forming Unit 9

Ⅳ. DISCUSSION 10

Ⅴ. CONCLUSIONS 14

Ⅵ. REFERENCES 15

Figure 17

Abstract in Korean 21
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dc.formatapplication/pdf-
dc.format.extent1304163 bytes-
dc.format.mediumapplication/pdf-
dc.language.isoen-
dc.publisher서울대학교 치의학대학원-
dc.subjectAnti-fungal efficacy-
dc.subjecthuman dentin block-
dc.subjectC. albicans biofilms-
dc.subjectsynthetic hu-man β-defensin-3-C15 peptide-
dc.subjectcolony forming unit-
dc.subject.ddc617-
dc.titleAntifungal efficacy of a synthetic human β-defensin-3-C15 against Candida albicans-infected dentin infection model-
dc.typeThesis-
dc.description.degreeMaster-
dc.citation.pages23-
dc.contributor.affiliation치의학대학원 치의학과-
dc.date.awarded2016-02-
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Theses (Master's Degree_치의학과)
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