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Mpp6 regulates the interaction of Nrd1 with nuclear exosome and the C-terminal domain of RNA polymerase II

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Authors
김규민
Advisor
이준호
Major
자연과학대학 생물물리 및 화학생물학과
Issue Date
2015
Publisher
서울대학교 대학원
Keywords
Nrd1 Mpp6 RNA중합효소II 전사종결 RNA가공
Description
학위논문 (석사)-- 서울대학교 대학원 : 생물물리 및 화학생물학과, 2015. 2. 이준호.
Abstract
The yeast saccharomyces cerevisiae Nrd1 interacts with C-terminal domain (CTD) of RNA polymerase II (RNApII) through its CTD-interacting domain (CID) and also associates with the nuclear exosome, thereby acting as both a transcription termination and RNA processing factor. It was recently reported that the Nrd1 CID couples RNApII termination and subsequent RNA processing by recruiting the nuclear exosome to the Nrd1 complex. However, what bridges the Nrd1 CID to the nuclear exosome was not clear. In this study using yeast two hybrid assay, I show two nuclear exosome cofactors, Mpp6 and Trf4 directly and competitively interact with the Nrd1 CID, and regulate the interaction of Nrd1 with RNApII and/or the exosome. Various analyses indicated that Mpp6 promotes the processing of Nrd1-terminated transcripts by Dis3, while Trf4 leads to Rrp6-dependent processing. It implies that Mpp6 and Trf4 may play pivotal roles in choosing a particular RNA processing route within the exosome by guiding the Nrd1-terminated transcripts to their preferred exonucleases.
Language
English
URI
http://hdl.handle.net/10371/131529
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College of Natural Sciences (자연과학대학)Biophysics and Chemical Biology (생물물리 및 화학생물학과)Theses (Master's Degree_생물물리 및 화학생물학과)
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