Global analysis of Yor289w interactome in Saccharomyces cerevisiae

Abstract

Identifying protein-protein interactions is essential for understanding protein function, since most cellular processes are mediated by interaction of proteins. Therefore, by identifying the interactome of a protein, we could determine the function of the protein. In this study, we conducted a genome-wide bimolecular fluorescent complementation (BiFC) assay in Saccharomyces cerevisiae to detect the protein-protein interactions of Yor289w and thus identify its functional role. A pRPL7B-VN-YOR289W strain and strains from a VC fusion library were mated and BiFC signals were visualized. Subsequent to BiFC screening and elimination of false positive signals, a total 56 candidates exhibited interactions with Yor289w. In the analysis of interactome, we found candidates clustered into processes related to translation initiation and nucleobase-containing small molecule metabolic processes. In addition we identified Yor289w protein expression increased in response to unfolded protein response (UPR), and that this increase was dependent on Hac1 and Ire1, which are key factors in UPR. This indicates Yor289w expression is under control of Hac1 and Ire1. On both genome-wide screening and expression data, we concluded that Yor289w might be involved in translational regulation under UPR conditions.