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Photoaging-associated changes in epidermal proliferative cell fractions in vivo

Cited 24 time in Web of Science Cited 28 time in Scopus
Authors
Kwon, Oh Sang; Yoo, Hyeon Gyeong; Han, Ji Hyun; Lee, Se Rah; Chung, Jin Ho; Eun, Hee Chul
Issue Date
2007-10-31
Publisher
Springer Verlag
Citation
Arch Dermatol Res. 2008 Jan;300(1):47-52. Epub 2007 Oct 30.
Keywords
AdultAgedAged, 80 and overAntigens, CD29/metabolismBiological Markers/metabolismBiopsyCell Differentiation/radiation effectsCell Proliferation/*radiation effectsEpidermis/metabolism/*pathology/radiation effectsHumansKeratinocytes/metabolism/*pathology/radiation effectsMaleProtein Precursors/metabolismSkin Aging/*pathology/radiation effectsStem Cells/metabolism/*pathology/radiation effectsUltraviolet Rays/adverse effects
Abstract
The epidermis is a dynamic epithelium with constant renewal throughout life. Epidermal homeostasis depends on two types of proliferative cells, keratinocyte stem cells (KSCs), and transit amplifying (TA) cells. In the case of chronologic aging, levels of KSCs tend to decrease and change functionally. However, little is known about the effect of photoaging on epidermal proliferative subtype populations. The aim of this study was to validate involucrin/beta1-integrin ratio as a molecular marker of epidermal photoaging, and to investigate the effects of photoaging caused by chronic UV exposure on the proliferative subtype populations. A total of 15 male volunteers (age range 20-24 and 77-85 years, Fitzpatrick skin phototype III-IV) provided sun-exposed and sun-protected skin samples for real-time RT-PCR, Western blot analysis and immunostaining. Fractional changes in proliferative subtype populations in photoaged and chronologically aged skins were analyzed by flow cytometry. The expression of beta1-integrin was found to be significantly reduced in photoaged skin and ratios of the expressions of involucrin to beta1-integrin were increased 2.6-fold only in elderly subjects. Interestingly, immunostaining of the sun-exposed skins of elderly subjects showed aberrant beta1-integrin expression over the basal layer and greater numbers of Ki-67-positive cells than in sun-protected buttock skin. Flow cytometric analysis revealed that the proportion of KSCs to TA cells was reversed in sun-exposed and sun-protected skins of elderly subjects. Our results suggest that KSC numbers may be lower in photoaged skin than in chronologically aged skin and could be applied to hyperplastic pattern of photoaging. These findings suggest that the epidermis of photoaged skin is impaired in terms of its proliferative potential by attempting to repair chronic UV exposure and that photoaging may be associated with alteration in the two proliferative cell fractions.
ISSN
0340-3696 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17968567

http://hdl.handle.net/10371/13175
DOI
https://doi.org/10.1007/s00403-007-0812-3
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Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dermatology (피부과학전공)Journal Papers (저널논문_피부과학전공)
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