Publications
Detailed Information
Investigation of the anti-inflammatory effect of GV1001, a peptide derived from human telomerase reverse transcriptase (hTERT) sequence : 사람 Telomerase 역전사효소 서열 유래 펩타이드 GV1001의 항염 효능에 대한 연구
Cited 0 time in
Web of Science
Cited 0 time in Scopus
- Authors
- Advisor
- 강재승
- Major
- 의과대학 의학과
- Issue Date
- 2015-08
- Publisher
- 서울대학교 대학원
- Keywords
- inflammation ; GV1001 ; enolase1 ; rheumatoid arthritis ; p38 MAPK ; NF-κB
- Description
- 학위논문 (석사)-- 서울대학교 대학원 : 의학과(해부학 전공), 2015. 8. 강재승.
- Abstract
- GV1001 is a peptide derived from human telomerase reverse transcriptase (hTERT) sequence, and has anti-cancer and anti-inflammatory effect. Enolase1 (ENO1) is a glycolytic enzyme and its stimulation induces to produce a large amount of pro-inflammatory cytokines from concanavalin (Con) A -activated peripheral blood mononuclear cells (PBMCs) and from ENO1 expressing monocytes and macrophages from rheumatoid arthritis (RA) patients. However, it is still unknown whether GV1001 could regulate the ENO1-mediated pro-inflammatory cytokines production. Therefore, I investigated whether GV1001 regulates ENO1-mediated pro-inflammatory cytokines production as an anti-inflammatory peptide. First, I found that GV1001 does not affect the expression of ENO1 on Con A-activated PBMCs and RA PBMCs. However, ENO1 stimulation increased the production of pro-inflammatory cytokines (TNF-α, IL-1β and IL-6) from Con A-activated PBMCs. And it is down-regulated by the pre-treatment of GV1001. GV1001 also decreases the production of pro-inflammatory cytokines from ENO1 stimulated RA PBMCs. And then I examined what kinds of signaling molecules are involved in the down-regulation of ENO1-mediated pro-inflammatory cytokine production by GV1001. When ENO1 on the surface of Con A-activated PBMCs and RA PBMCs is stimulated, I found that p38 MAPK and NF-κB are activated. However, these are successfully suppressed by the pre-treatment of GV1001. Taken together, GV1001 might be a useful anti-inflammatory peptide via the down-regulation of pro-inflammatory cytokine production and the suppression of the p38 MAPK and NF-κB activation by ENO1 stimulation.
- Language
- English
- Files in This Item:
- Appears in Collections:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.