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Identification of the human cytomegalovirus in Korean glioblastoma patients : 한국인의 교모세포종에서 거대세포바이러스 항원단백질 발현 확인

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Authors

이경열

Advisor
최기영
Major
의과대학 의학과
Issue Date
2016-02
Publisher
서울대학교 대학원
Keywords
glioblastomaHCMV
Description
학위논문 (석사)-- 서울대학교 대학원 : 의학과 중개의학, 2016. 2. 최기영.
Abstract
Glioblastoma multiforme (GBM) is the most frequent brain tumor with a median overall survival of less than 2 years. Recent studies indicate that most of GBM tumors are associated with the human cytomegalovirus (HCMV). However, the presence of HCMV in GBM is still controversial because a minority of studies failed to identify the evidence of HCMV in GBM until recently. Furthermore, the evidence of HCMV in Asian GBM patients has never been reported. The aim of the study is to find the evidence of HCMV in Korean GMB tumors. We collected 98 cases of GBM from 2004 to 2012. Tissue microarray blocks with 3 mm cores were constructed using formalin-fixed, paraffin-embedded archival tissue blocks. To detect the low level expression of HCMV antigen in GBM tumors, we fulfilled the essential immunohistochemistry procedures proposed by the first reporting group. We used two antibodies for HCMV pp65 antigen and HCMV EA antigen. The HCMV pp65 antigen was detected in 73/90 (81.1%) and HCMV EA antigen was found in 46/90 (51.1%) of GBM cases. The intensity of staining was weak or moderate due to the low level antigen expression. Moderate intensity was found in 17/90 (18.9%) for pp65 and 12/90 (13.3%) for EA. Co-expression of HCMV pp65 and EA was noted in 41/90 (45.6%). In conclusion, the evidence of HCMV in Asian GBM tumors was identified for the first time.
Language
Korean
URI
https://hdl.handle.net/10371/132836
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