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Cytokine responses of PG102, an aqueous extract of Actinidia arguta fruit, on PBMCs from dogs with canine atopic dermatitis : 아토피 피부염에 이환된 개의 PBMC에서 다래추출물 PG102의 Cytokine 조절 효과

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Authors

Yong-Uk Lee

Advisor
황철용
Major
수의과대학 수의학과
Issue Date
2013-02
Publisher
서울대학교 대학원
Keywords
PG102Actinidia argutacanine atopic dermatitisPBMCcytokine
Description
학위논문 (석사)-- 서울대학교 대학원 : 수의학과(수의내과학(피부과학)전공), 2013. 2. 황철용.
Abstract
An aqueous extract, PG102, derived from Actinidia arguta fruit, has been investigated as an anti-inflammatory agent in an animal model, humans, and dog atopy patients. However, its mechanism of action is not well understood. This study evaluated the in vitro effects of PG102 on canine peripheral blood mononuclear cells (PBMCs). PBMCs were isolated from eight dogs with atopic dermatitis and stimulated with house dust mite extract (DME) for 12 hours in the presence of PG102 (0.1, 1, 10, 100 μg/ml). Subsequent quantitative real-time PCR revealed changes in mRNA expressions of representative allergy-related cytokines, such as proinflammatory cytokines (TNF-α), Th1 cell cytokines (IFN-γ), Th2 cell cytokines (IL-4, IL-5, IL-13), and regulatory cytokines (IL-10 and TGF-β1) calculated by 2-ΔΔCT and expressed as n-fold differences compared with an unstimulated control.
Cytokine suppression by PG102 was compared with that of Dexamethasone, a widely used drug for the control of symptoms of canine atopic dermatitis. PG102 significantly inhibited the expression of TNF-α, IFN-γ and IL-4, IL-5, IL-13, but not the expression of regulatory cytokines (IL-10 and TGF-β1), when compared to a DME stimulated sample (P<0.05). This pattern was similar to that obtained with Dexamethasone except for TGF-β1. No significant reduction was seen with 100 μg/ml PG102
however, treatment with concentrations of 1 and 10 μg/ml significantly reduced cytokine expression, indicating that 1 and 10 μg/ml of PG102 were effective concentrations for suppressing cytokines in PBMCs. PG102 exerts an anti-inflammatory effect through the inhibition of cytokine production by PBMCs, indicating that it has potential as an alternative therapy for treatment of canine atopic dermatitis.
Language
English
URI
https://hdl.handle.net/10371/133687
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Theses (Master's Degree_수의학과)
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