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Effect of adipose tissue -derived mesenchymal stem cells engineering with Oct4/Sox2 on inflammatory mouse models
Oct4/Sox2 과발현 지방유래중간엽줄기세포의 마우스 염증 모델에 미치는 영향

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Authors
이강
Advisor
윤화영
Major
수의과대학 수의학과
Issue Date
2015-08
Publisher
서울대학교 대학원
Keywords
inflammationIL-2IL-6IL-10mesenchymal stem cellOct4/Sox2
Description
학위논문 (석사)-- 서울대학교 대학원 : 수의학과(수의내과학 전공), 2015. 8. 윤화영.
Abstract
Inflammation is part of a complex biological defense against harmful stimuli. When inflammatory cytokines are released, they induce redness, swelling, and heat
multi-organ failure is possibly the result of cytokine overexpression. The immune modulating effect of adipose tissue derived mesenchymal stem cells (ATMSCs) has been known to change the immune response by altering inflammatory cytokine secretion. In this study, we evaluated the anti-inflammatory effects of Oct4/Sox2 engineered mesenchymal stem cells using reverse transcription polymerase chain reaction (RT-PCR) and immunostaining, to confirm the successful transduction of Oct4/Sox2 in ATMSCs. GFP (control) and Oct4/Sox2-ATMSCs were cultured for 48 h and the supernatant (conditioned media) was collected to treat lipopolysaccharide (LPS)-induced Raw 264.7 cells (mouse macrophage-like cells). Subsequently, the levels of the inflammatory cytokines, Interleukin (IL)-2, IL-6, and IL-10 were determined using RT-PCR analysis. Comparison of GFP- and Oct4/Sox2-ATMSCs conditioned media-treatment and the LPS stimulated group revealed 2- and 5.8-fold reduction in IL-2 expression, respectively, and 1.08- and 1.68-fold reduction in IL-6 expression, respectively. Additionally, macrophages treated with GFP- and Oct4/Sox2-ATMSCs conditioned media expressed similar levels of IL-10. Mice were stimulated with LPS (20mg/kg) and were evaluated with a sick score based on symptoms such as, diarrhea, eye condition, and the condition of their fur. Compared to the phosphate buffer saline (PBS)-injected group with GFP and Oct4/Sox2-ATMSCs, the total sick score was reduced to 1.23- and 1.89-fold, while the survival rate was increased by 5.6% and 16.7%. These results suggest that Oct4/Sox2-ATMSCs have superior inflammatory modulation effects than GFP-ATMSCs. According to this result, the Oct4/Sox2-ATMSCs anti-inflammatory effect may be applied to a variety of inflammatory-related diseases in veterinary medicine
Language
English
URI
http://hdl.handle.net/10371/133735
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Theses (Master's Degree_수의학과)
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