Antitumor Effects of SB Injection, Extract from Phytopharmaceutical Preparations, in Canine Osteosarcoma and Melanoma Cell Lines

Abstract

The purpose of this study is to evaluate the effects of SB injection, an antitumor agent obtained from natural extracts, on canine cells. The antitumor effect of SB injection in D17 (canine osteosarcoma cells) and LMeC (canine melanoma cells) was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, cell cycle analysis, and annexin-V assay. Cell viability was inversely related to the concentration of chemical agents. The cell cycle of the affected cells was arrested in the G2/M phase that led to an anti-proliferation effect. SB increased the rate of apoptosis dose-dependently. It is also used in combination with chemical drugs to reduce its dosage. SB showed no effect on the viability of PBMC (peripheral blood mononuclear cells) regardless of concentration, which suggested that SB does not suppress the activity of normal blood cells. This study suggests possibility of SB that can be considered as an alternative medication for canine osteosarcoma and melanoma in veterinary medicine.