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Transcriptomics and methylomics in chronic periodontitis with tobacco use: a pilot study

Cited 7 time in Web of Science Cited 8 time in Scopus
Authors
Cho, Young-Dan; Kim, Pil-Jong; Kim, Hong-Gee; Seol, Yang-Jo; Lee, Yong-Moo; Ku, Young; Rhyu, In-Chul; Ryoo, Hyun-Mo
Issue Date
2017-08-10
Publisher
BioMed Central
Citation
Clinical Epigenetics, 9(1):81
Keywords
DNA methylationEpigenomicsExtracellular matrixPeriodontal diseaseSmokingTranscriptome
Description
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Abstract
Abstract

Background
Accumulating evidence suggests that tobacco smoking affects the susceptibility to and severity of chronic periodontitis. Epigenetics may explain the role of smoking in the development and progress of periodontal disease. In this study, we performed transcriptomic and methylomic analyses of non-periodontitis and periodontitis-affected gingival tissues according to smoking status.

Methods
Human gingival tissues were obtained from 20 patients, including non-smokers with and without periodontitis (n = 5 per group) and smokers with and without periodontitis (n = 5 per group). Total RNA and genomic DNA were isolated, and their quality was validated according to strict standards. The Illumina NextSeq500 sequencing system was used to generate transcriptome and methylome datasets.

Results
Comprehensive analysis, including between-group correlation, differential gene expression, DNA methylation, gene set enrichment, and protein-protein interaction, indicated that smoking may change the transcription and methylation states of extracellular matrix (ECM) organization-related genes, which exacerbated the periodontal condition.

Conclusions
Our results suggest that smoking-related changes in DNA methylation patterns and subsequent alterations in the expression of genes coding for ECM components may be causally related to the increased susceptibility to periodontitis in smokers as they could influence ECM organization, which in turn may have an effect on disease characteristics.
ISSN
1868-7083
Language
English
URI
http://hdl.handle.net/10371/137258
DOI
https://doi.org/10.1186/s13148-017-0381-z
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dental Research Institute (치학연구소)Journal Papers (저널논문_치학연구소)
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