Browse

Good correlation of HPV DNA test between self-collected vaginal and clinician-collected cervical samples by the oligonucleotide microarray

Cited 21 time in Web of Science Cited 21 time in Scopus
Authors
Seo, Sang-Soo; Song, Yong-Sang; Kim, Jae-Weon; Park, Noh-Hyun; Kang, Soon-Beom; Lee, Hyo-Pyo
Issue Date
2005-12-27
Publisher
Elsevier
Citation
Gynecol Oncol. 2006 Jul;102(1):67-73. Epub 2006 Jan 10.
Keywords
AdolescentAdultAgedCervix Uteri/cytology/*virologyDNA, Viral/*geneticsFemaleHumansMiddle AgedOligonucleotide Array Sequence Analysis/methodsRisk FactorsSelf Care/methodsUterine Cervical Neoplasms/pathology/*virologyVaginal Smears/*methods
Abstract
OBJECTIVES: To evaluate the efficacy of self-collected vaginal samples for high-risk HPV detection by the HPV oligonucleotide microarray method (HPVDNAChip). METHODS: One hundred and eighteen patients with abnormal Pap smears were included. Self-collected vaginal and clinician-collected cervical samples for HPV testing were obtained. The result of the HPV DNA test was compared with the histopathological diagnosis or colposcopic finding. RESULTS: Of the 118 patients, 42 (35.6%) had >or= cervical intraepithelial neoplasia (CIN) III lesions. Using the HPVDNAChip, high-risk types of HPV were detected in 38 of these 42 patients (90.5%) with the self-collected vaginal samples and in 37 of 42 (88.1%) with the clinician-collected cervical samples. The agreement of HPVDNAchip results between self- and clinician-collected samples was very good (kappa = 0.81) with a 93.2% concordance rate. Multiple HPV infections were found in 17 of 88 (19.3%) HPV-positive clinician-collected cervical samples. The rate of multiple HPV infection tended to decrease as the degree of pathologic classification increased. CONCLUSION: Using the HPVDNAchip to assay for HPV infection, results from self-collected vaginal samples were compatible with those from clinician-collected cervical samples.
ISSN
0090-8258 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16375952

http://hdl.handle.net/10371/13770
DOI
https://doi.org/10.1016/j.ygyno.2005.11.030
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Obstetrics & Gynecology (산부인과전공)Journal Papers (저널논문_산부인과학전공)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse