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Effect of canine cortical bone demineralization on osteogenic differentiation of adipose-derived mesenchymal stromal cells
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- Authors
- Advisor
- 권오경
- Major
- 수의과대학 수의학과
- Issue Date
- 2017-08
- Publisher
- 서울대학교 대학원
- Keywords
- Osteogenesis ; allograft ; demineralization degree ; adipose-derived mesenchymal stromal cells ; dogs
- Description
- 학위논문 (석사)-- 서울대학교 대학원 수의과대학 수의학과, 2017. 8. 권오경.
- Abstract
- Demineralized bone allografts and mesenchymal stromal cells have been used to promote bone regeneration. However, the degree to which cortical bone should be demineralized for use in combination with adipose-derived mesenchymal stromal cells (Ad-MSCs) remains to be clarified. In this study, the in vitro osteogenic ability of Ad-MSCs on allografts was investigated in relation to the extent of demineralization. Three treatment groups were established by varying exposure time to 0.6 N HCL: partially demineralized (PDB
12 h), fully demineralized (FDB
48 h), and non-demineralized bone (NDB
0 h, as a control). Allografts were prepared as discs 6 mm in diameter for in vitro evaluation, and their demineralization and structure were evaluated by micro-computed tomography and scanning electron microscopy. Ad-MSC adhesion and proliferation were measured by MTS assay, and osteogenesis-related gene expression was assessed by quantitative reverse transcription polymerase chain reaction. PDB and FDB demineralization rates were 57.13 and 92.30 %, respectively. Moreover, Ad-MSC adhesion rates on NDB, PDB, and FDB were 53.41, 60.65, and 61.32 %, respectively. Proliferation of these cells on FDB increased significantly after 2 days of culture compared to the other groups (P < 0.05). Furthermore, expression of the osteogenic genes ALP, BMP-7, and TGF-β in the FDB group on culture day 3 was significantly elevated in comparison to the other treatments. Given its biocompatibility and promotion of the osteogenic differentiation of Ad-MSCs, our results suggest that FDB may be a suitable scaffold for use in the repair of bone defects.
- Language
- English
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