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Dynamic changes of oligomeric amyloid β levels in plasma induced by spiked synthetic Aβ42

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Authors
An, Seong Soo A.; Lee, Byoung-sub; Yu, Ji Sun; Lim, Kuntaek; Kim, Gwang Je; Lee, Ryan; Kim, Shinwon; Kang, Sungmin; Park, Young Ho; Wang, Min Jeong; Yang, Young Soon; Youn, Young Chul; Kim, SangYun
Issue Date
2017-10-17
Publisher
BioMed Central
Citation
Alzheimer's Research & Therapy, 9(1):86
Keywords
Multimer detection systemAlzheimer’s diseaseAmyloid-βOligomersBlood biomarkerSynthetic amyloid-βELISAPlasma test
Abstract
Abstract

Background
A reliable blood-based assay is required to properly diagnose and monitor Alzheimer’s disease (AD). Many attempts have been made to develop such a diagnostic tool by measuring amyloid-β oligomers (AβOs) in the blood, but none have been successful in terms of method reliability. We present a multimer detection system (MDS), initially developed for the detection of prion oligomers in the blood, to detect AβOs.

Methods
To characterize Aβ in the blood, plasma was spiked with synthetic amyloid-β (Aβ) and incubated over time. Then, the MDS was used to monitor the dynamic changes of AβO levels in the plasma.

Results
Increasing concentrations of AβOs were observed in the plasma of patients with AD but not in the plasma of normal control subjects. The plasma from patients with AD (n = 27) was differentiated from that of the age-matched normal control subjects (n = 144) with a sensitivity of 83.3% and a specificity of 90.0%.

Conclusions
Synthetic Aβ spiked into the blood plasma of patients with AD, but that of not elderly normal control subjects, induced dynamic changes in the formation of AβOs over time. AβOs were detected by the MDS, which is a useful blood-based assay with high sensitivity and specificity for AD diagnosis.
ISSN
1758-9193
Language
English
URI
http://hdl.handle.net/10371/138334
DOI
https://doi.org/10.1186/s13195-017-0310-6
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Neurology (신경과학교실)Journal Papers (저널논문_신경과학교실)
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