S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Neurology (신경과학교실) Journal Papers (저널논문_신경과학교실)
Dynamic changes of oligomeric amyloid β levels in plasma induced by spiked synthetic Aβ42
- An, Seong Soo A.; Lee, Byoung-sub; Yu, Ji Sun; Lim, Kuntaek; Kim, Gwang Je; Lee, Ryan; Kim, Shinwon; Kang, Sungmin; Park, Young Ho; Wang, Min Jeong; Yang, Young Soon; Youn, Young Chul; Kim, SangYun
- Issue Date
- BioMed Central
- Alzheimer's Research & Therapy, 9(1):86
- Multimer detection system; Alzheimer’s disease; Amyloid-β; Oligomers; Blood biomarker; Synthetic amyloid-β; ELISA; Plasma test
A reliable blood-based assay is required to properly diagnose and monitor Alzheimer’s disease (AD). Many attempts have been made to develop such a diagnostic tool by measuring amyloid-β oligomers (AβOs) in the blood, but none have been successful in terms of method reliability. We present a multimer detection system (MDS), initially developed for the detection of prion oligomers in the blood, to detect AβOs.
To characterize Aβ in the blood, plasma was spiked with synthetic amyloid-β (Aβ) and incubated over time. Then, the MDS was used to monitor the dynamic changes of AβO levels in the plasma.
Increasing concentrations of AβOs were observed in the plasma of patients with AD but not in the plasma of normal control subjects. The plasma from patients with AD (n = 27) was differentiated from that of the age-matched normal control subjects (n = 144) with a sensitivity of 83.3% and a specificity of 90.0%.
Synthetic Aβ spiked into the blood plasma of patients with AD, but that of not elderly normal control subjects, induced dynamic changes in the formation of AβOs over time. AβOs were detected by the MDS, which is a useful blood-based assay with high sensitivity and specificity for AD diagnosis.