S-Space Graduate School of International Agricultural Technology (국제농업기술대학원) Dept. of International Agricultural Technology (국제농업기술학과) Journal Papers (저널논문_국제농업기술학과)
Transglycosylation of gallic acid by using Leuconostoc glucansucrase and its characterization as a functional cosmetic agent
- Nam, Seung-Hee; Park, Jeongjin; Jun, Woojin; Kim, Doman; Ko, Jin-A; Abd El-Aty, A. M.; Choi, Jin Young; Kim, Do-Ik; Yang, Kwang-Yeol
- Issue Date
- Springer Open
- AMB Express, 22;7(1):224
- Leuconostoc mesenteroides; Dextransucrase; Gallic acid; Glucosylation; Whitening efect; Anti-aging efect
Gallic acid glycoside was enzymatically synthesized by using dextransucrase and sucrose from gallic acid. After purification by butanol partitioning and preparative HPLC, gallic acid glucoside was detected at m/z 355 (C13, H16, O10, Na)+ by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The yield of gallic acid glucoside was found to be 35.7% (114 mM) by response surface methodology using a reaction mixture of 319 mM gallic acid, 355 mM sucrose, and 930 mU/mL dextransucrase. The gallic acid glucoside obtained showed 31% higher anti-lipid peroxidation and stronger inhibition (Ki = 1.23 mM) against tyrosinase than that shown by gallic acid (Ki = 1.98 mM). In UVB-irradiated human fibroblast cells, gallic acid glucoside lowered matrix metalloproteinase-1 levels and increased the collagen content, which was indicative of a stronger anti-aging effect than that of gallic acid or arbutin. These results indicated that gallic acid glucoside is likely a superior cosmetic ingredient with skin-whitening and anti-aging functions.