Studies on application of primordial germ cells for interspecies chimera and molecular characterization of NANOG in aves

Abstract

Avian is one of the excellent models for basic and applied research such as reproductive system and developmental biology. In particular, avian primordial germ cells (PGCs) have unique characteristics and accessibility compared to mammals during early embryo development. Thus, the production of germline chimera has been regarded as the most powerful tool via PGCs-mediated technology. In addition, a detailed understanding of germ cells has been emphasized for maintaining distinct properties in germ cell biology. In this regards, we achieved the combination of the unique migratory route of avian germ cells with an embryo replacement method could be applied to produce interspecies germline chimeras and developed potential knowledge for the conservation of wild and endangered birds through surrogate system. Furthermore, we investigated the structural characteristics of chicken NANOG (cNANOG) protein and its biological significance using biochemical methods and cell assay in PGCs. Also, we revealed the oviduct specific genes regulated by estrogen for potential therapeutic peptides or regulation of egg white protein secretion.