Publications

Detailed Information

HBNP Incorporated, Aligned Nanofiber Scaffolds for Osteogenesis : 골재생을 위한 말뼈 분말이 포함된 방향성 나노섬유 스캐폴드

DC Field Value Language
dc.contributor.advisor정종훈-
dc.contributor.author임재운-
dc.date.accessioned2018-05-29T03:55:29Z-
dc.date.available2018-05-29T03:55:29Z-
dc.date.issued2018-02-
dc.identifier.other000000149942-
dc.identifier.urihttps://hdl.handle.net/10371/141781-
dc.description학위논문 (석사)-- 서울대학교 대학원 : 농업생명과학대학 바이오시스템·소재학부, 2018. 2. 정종훈.-
dc.description.abstractVarious scaffolds have been attempted on osteogenesis and osteogenic differentiation. Recently poly (ε-caprolactone) has been employed in various in vivo medical devices. This semi-crystalline polymer have been combined with other polymers or additives to improve various properties. It was also used to fabricate effective nanostructures using various methods such as lithography and electrospinning. Several approaches that add nanosized hydroxyapatite (nHA) into electrospun PCL nanofibers showed great performances. However, the nHA used to the previous studies are synthetic HA, because nanosized xenogenic HA have not been developed. Recently, we developed new horse bone-derived nanopowder (HBNP) from waste horse bone. Because xenogenic HA has better osteogenic ability than synthetic HA, HBNP-incorporated nanofibrous scaffold is anticipated to have better healing efficacy than formerly developed HA-incorporated nanofibrous scaffolds. In this study, we developed electrospun, HBNP-incorporated nanofibrous scaffolds (HBNP-NSs). Furthermore, aligned HBNP-NSs as well as random HBNP-NSs were fabricated using rotating drum. Eight kinds of specimen were prepared by varying HBNP/nHA percentage in PCL solution. Scanning electron microscopy showed that fibers produced using rotating drum are well aligned to certain direction. As high resolution transmission electron microscopy (HFTEM) suggests, HBNP and nHA particles are well incorporated into the fiber. To determine the effect of HBNP and aligned nanofiber structure on bone progenitor cell, dental pulp stem cells (DPSCs) were cultured on the NSs and analyzed by WST assay. From proliferation test, it turned out that aligned, HBNP incorporated scaffold promoted proliferation and viability of DPSCs. In future study, we are looking forward to carry on additional differentiation test and in vivo proliferation and differentiation test in the end.-
dc.description.tableofcontents1. Introduction 1
2. Objectives 5
3. Literature Review - 6
3.1 Characteristics of PCL and making scaffolds 6
3.2 Electrospun nanofiber structure - 7
3.3 Improvement of PCL electrospun nanofiber scaffold 8
3.3.1 Aligned structure 8
3.3.2 nHA addition, coating and mineralization 9
3.3.3 HBNP as nHA substitute 10
4. Material and Methods - 11
4.1 Cell selection and culture 11
4.2 Scaffold fabrication 11
4.2.1 PCL Solution - 11
4.2.2 PDMS - 11
4.2.3. Sample making 12
4.2.3.1 Random scaffold - 12 4.2.3.2 Aligned scaffold - 13
4.2.4 Nanofiber with PDMS platform - 14
4.3 Characterization of nanofiber scaffolds by FESEM, TEM - 15
4.4 Determining chemical properties of nanofiber scaffolds by XRD - 15
4.5 Determining cell viability and proliferation on the scaffold in vitro 15
4.5.1. Cell culture on the scaffold 15
4.5.1.1 Culture media 16
4.5.2 WST assay - 16
4.6 Determining differentiation on the scaffold in vitro - 17
4.6.1 Alizarin red staining - 17
4.6.2 Immunocytochemistry 18
4.7 Statics Analysis 19
5. Results 20
5.1 Characterization of nanofiber scaffolds - 20
5.1.1 SEM results and fiber orientation analysis - 20
5.1.2 TEM results 23
5.1.3 XRD results 23
5.2 In vitro results 24
5.2.1 Cell viability tests - 24
5.2.2 Differentiation tests - 26
6. Discussion - 34
7. References - 36
Abstract in Korean 38
-
dc.formatapplication/pdf-
dc.format.extent2001051 bytes-
dc.format.mediumapplication/pdf-
dc.language.isoen-
dc.publisher서울대학교 대학원-
dc.subjectTissue engineering-
dc.subjectOsteogenesis-
dc.subjectBioceramic-
dc.subjectHorse bone-
dc.subject.ddc660.6-
dc.titleHBNP Incorporated, Aligned Nanofiber Scaffolds for Osteogenesis-
dc.title.alternative골재생을 위한 말뼈 분말이 포함된 방향성 나노섬유 스캐폴드-
dc.typeThesis-
dc.contributor.AlternativeAuthorLim, Jae Woon-
dc.description.degreeMaster-
dc.contributor.affiliation농업생명과학대학 바이오시스템·소재학부-
dc.date.awarded2018-02-
Appears in Collections:
Files in This Item:

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share