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M1 macrophage recruitment correlates with worse outcome in SHH Medulloblastomas
Cited 35 time in
Web of Science
Cited 35 time in Scopus
- Authors
- Issue Date
- 2018-05-08
- Publisher
- BMC
- Citation
- BMC Cancer, 18(1):535
- Keywords
- Medulloblastoma ; Sonic hedgehog ; Macrophage ; Recruitment ; Prognosis
- Abstract
- Background
Recent progress in molecular analysis has advanced the understanding of medulloblastoma (MB) and is anticipated to facilitate management of the disease. MB is composed of 4 molecular subgroups: WNT, SHH, Group 3, and Group 4. Macrophages play a crucial role in the tumor microenvironment; however, the functional role of their activated phenotype (M1/M2) remains controversial. Herein, we investigate the correlation between tumor-associated macrophage (TAM) recruitment within the MB subgroups and prognosis.
Methods
Molecular subgrouping was performed by a nanoString-based RNA assay on retrieved snap-frozen tissue samples. Immunohistochemistry (IHC) and immunofluorescence (IF) assays were performed on subgroup identified samples, and the number of polarized macrophages was quantified from IHC. Survival analyses were conducted on collected clinical data and quantified macrophage data.
Results
TAM (M1/M2) recruitment in SHH MB was significantly higher compared to that in other subgroups. A Kaplan-Meier survival curve and multivariate Cox regression demonstrated that high M1 expressers showed worse overall survival (OS) and progression-free survival (PFS) than low M1 expressers in SHH MB, with relative risk (RR) values of 11.918 and 6.022, respectively.
Conclusion
M1 rather than M2 correlates more strongly with worse outcome in SHH medulloblastoma.
- ISSN
- 1471-2407
- Language
- English
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