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Hypoxic condition enhances chondrogenesis in synovium-derived mesenchymal stem cells

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dc.contributor.authorBae, Hyun Cheol-
dc.contributor.authorPark, Hee Jung-
dc.contributor.authorWang, Sun Young-
dc.contributor.authorYang, Ha Ru-
dc.contributor.authorLee, Myung Chul-
dc.contributor.authorHan, Hyuk-Soo-
dc.date.accessioned2019-01-17T01:04:55Z-
dc.date.available2019-01-17T10:12:17Z-
dc.date.issued2018-09-26-
dc.identifier.citationBiomaterials Research, 22(1):28ko_KR
dc.identifier.issn2055-7124-
dc.identifier.urihttp://hdl.handle.net/10371/145159-
dc.description.abstractBackground
The chondrogenic differentiation of mesenchymal stem cells (MSCs) is regulated by many factors, including oxygen tensions, growth factors, and cytokines. Evidences have suggested that low oxygen tension seems to be an important regulatory factor in the proliferation and chondrogenic differentiation in various MSCs. Recent studies report that synovium-derived mesenchymal stem cells (SDSCs) are a potential source of stem cells for the repair of articular cartilage defects. But, the effect of low oxygen tension on the proliferation and chondrogenic differentiation in SDSCs has not characterized. In this study, we investigated the effects of hypoxia on proliferation and chondrogenesis in SDSCs.

Method
SDSCs were isolated from patients with osteoarthritis at total knee replacement. To determine the effect of oxygen tension on proliferation and colony-forming characteristics of SDSCs, A colony-forming unit (CFU) assay and cell counting-based proliferation assay were performed under normoxic (21% oxygen) or hypoxic (5% oxygen). For in vitro chondrogenic differentiation, SDSCs were concentrated to form pellets and subjected to conditions appropriate for chondrogenic differentiation under normoxia and hypoxia, followed by the analysis for the expression of genes and proteins of chondrogenesis. qRT-PCR, histological assay, and glycosoaminoglycan assays were determined to assess chondrogenesis.

Results
Low oxygen condition significantly increased proliferation and colony-forming characteristics of SDSCs compared to that of SDSCs under normoxic culture. Similar pellet size and weight were found for chondrogensis period under hypoxia and normoxia condition. The mRNA expression of types II collagen, aggrecan, and the transcription factor SOX9 was increased under hypoxia condition. Histological sections stained with Safranin-O demonstrated that hypoxic conditions had increased proteoglycan synthesis. Immunohistochemistry for types II collagen demonstrated that hypoxic culture of SDSCs increased type II collagen expression. In addition, GAG deposition was significantly higher in hypoxia compared with normoxia at 21 days of differentiation.

Conclusion
These findings show that hypoxia condition has an important role in regulating the synthesis ECM matrix by SDSCs as they undergo chondrogenesis. This has important implications for cartilage tissue engineering applications of SDSCs.
ko_KR
dc.description.sponsorshipThis work was supported by Seoul National University Hospital Research Fund (Grant numbers: 06–2018-0918), Republic of Korea.ko_KR
dc.language.isoenko_KR
dc.publisherBioMed Centralko_KR
dc.subjectSynovium-derived mesenchymal stem cellsko_KR
dc.subjectSDSCsko_KR
dc.subjectHypoxiako_KR
dc.subjectChondrogensisko_KR
dc.subjectOxygenko_KR
dc.titleHypoxic condition enhances chondrogenesis in synovium-derived mesenchymal stem cellsko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor배현철-
dc.contributor.AlternativeAuthor박희정-
dc.contributor.AlternativeAuthor왕선영-
dc.contributor.AlternativeAuthor양하루-
dc.contributor.AlternativeAuthor이명철-
dc.contributor.AlternativeAuthor한혁수-
dc.identifier.doi10.1186/s40824-018-0134-x-
dc.language.rfc3066en-
dc.rights.holderThe Author(s).-
dc.date.updated2018-09-30T03:57:55Z-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Orthopedic Surgery (정형외과학전공)Journal Papers (저널논문_정형외과학전공)
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