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Fibroblast growth factor-1 as a mediator of paracrine effects of canine adipose tissue-derived mesenchymal stem cells on in vitro-induced insulin resistance models

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dc.contributor.authorKim, Hyeon-Jin-
dc.contributor.authorLi, Qiang-
dc.contributor.authorSong, Woo-Jin-
dc.contributor.authorYang, Hye-Mi-
dc.contributor.authorKim, Su-Yeon-
dc.contributor.authorPark, Sang-Chul-
dc.contributor.authorAhn, Jin-Ok-
dc.contributor.authorYoun, Hwa-Young-
dc.date.accessioned2019-03-11T04:22:53Z-
dc.date.available2019-03-11T13:24:12Z-
dc.date.issued2018-11-16-
dc.identifier.citationBMC Veterinary Research, 14(1):351ko_KR
dc.identifier.issn1746-6148-
dc.identifier.urihttp://hdl.handle.net/10371/146929-
dc.description.abstractBackground
In the field of diabetes research, many studies on cell therapy have been conducted using mesenchymal stem cells. This research was intended to shed light on the influence of canine adipose-tissue-derived mesenchymal stem cell conditioned medium (cAT-MSC CM) on in vitro insulin resistance models that were induced in differentiated 3T3-L1 adipocytes and the possible mechanisms involved in the phenomenon.

Results
Gene expression levels of insulin receptor substrate-1 (IRS-1) and glucose transporter type 4 (GLUT4) were used as indicators of insulin resistance. Relative protein expression levels of IRS-1 and GLUT4 were augmented in the cAT-MSC CM treatment group compared to insulin resistance models, indicating beneficial effects of cAT-MSC to DM, probably by actions of secreting factors. With reference to previous studies on fibroblast growth factor-1 (FGF1), we proposed FGF1 as a key contributing factor to the mechanism of action. We added anti-FGF1 neutralizing antibody to the CM-treated insulin resistance models. As a result, significantly diminished protein levels of IRS-1 and GLUT4 were observed, supporting our assumption. Similar results were observed in glucose uptake assay.

Conclusions
Accordingly, this study advocated the potential of FGF-1 from cAT-MSC CM as an alternative insulin sensitizer and discovered a signalling factor associated with the paracrine effects of cAT-MSC.
ko_KR
dc.description.sponsorshipThis study was supported by the Research Institute for Veterinary Science and the BK21 PLUS Program for Creative Veterinary Science Research; this includes design of study, and collection, analysis, and interpretation of data, and writing of manuscript.ko_KR
dc.language.isoenko_KR
dc.publisherBioMed Centralko_KR
dc.subjectFibroblast growth factor-1ko_KR
dc.subjectCanineko_KR
dc.subjectAdipose tissueko_KR
dc.subjectMesenchymal stem cellsko_KR
dc.subjectConditioned mediumko_KR
dc.subjectDiabetes mellitusko_KR
dc.subjectInsulin resistanceko_KR
dc.subjectIn vitroko_KR
dc.subject3T3-L1ko_KR
dc.titleFibroblast growth factor-1 as a mediator of paracrine effects of canine adipose tissue-derived mesenchymal stem cells on in vitro-induced insulin resistance modelsko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor김현진-
dc.contributor.AlternativeAuthor송우진-
dc.contributor.AlternativeAuthor양혜미-
dc.contributor.AlternativeAuthor김수연-
dc.contributor.AlternativeAuthor박상철-
dc.contributor.AlternativeAuthor안진옥-
dc.contributor.AlternativeAuthor연화영-
dc.identifier.doi10.1186/s12917-018-1671-1-
dc.language.rfc3066en-
dc.rights.holderThe Author(s).-
dc.date.updated2018-11-18T04:27:48Z-
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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