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GPR119 agonist enhances gefitinib responsiveness through lactate-mediated inhibition of autophagy

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Authors
Im, Ji Hye; Kang, Keon Wook; Kim, Sun Young; Kim, Yoon Gyoon; An, Yong Jin; Park, Sunghyouk; Jeong, Byung Hwa; Choi, Song-Yi; Lee, Jin-Sun; Kang, Keon Wook
Issue Date
2018-11-29
Publisher
BioMed Central
Citation
Journal of Experimental & Clinical Cancer Research, 37(1):295
Keywords
AutophagyBreast cancerGefitinibGPR119 agonistLactate
Abstract
Background
Ligand-dependent activation of the G-protein coupled receptor 119 (GPR119) lowers blood glucose via glucose-dependent insulin secretion and intestinal glucagon-like peptide-1 production. However, the function of GPR119 in cancer cells has not been studied.

Methods
GPR119 expression was assessed by real-time qPCR and immunohistochemistry in human breast cancer cell lines and breast cancer tissues. Cell proliferation and cell cycle analyses were performed by Incucyte® live cell analysis system and flow cutometry, respectively. Autophagy activity was estimeated by western blottings and LC3-GFP transfection.

Results
mRNA or protein expression of GPR119 was detected in 9 cancer cell lines and 19 tissue samples. Cotreatment with GPR119 agonist (MBX-2982 or GSK1292263) significantly potentiated gefitinib-induced cell growth inhibition in gefitinib-insensitive MCF-7 and MDA-MB-231 breast cancer cells. We observed that caspase-3/7 activity was enhanced with the downregulation of Bcl-2 in MCF-7 cells exposed to MBX-2982. Gefitinib-induced autophagy is related with cancer cell survival and chemoresistance. GPR119 agonists inhibit gefitinib-induced autophagosome formation in MCF-7 and MDA-MB-231 cells. MBX-2982 also caused a metabolic shift to enhanced glycolysis accompanied by reduced mitochondrial oxidative phosphorylation. MBX-2982 increased intracellular (~ 2.5 mM) and extracellular lactate (~ 20 mM) content. Gefitinib-mediated autophagy was suppressed by 20 mM lactate in MCF-7 cells.

Conclusions
GPR119 agonists reduced mitochondrial OXPHOS and stimulated glycolysis in breast cancer cells, with consequent overproduction of lactate that inhibited autophagosome formation. Because autophagy is crucial for the survival of cancer cells exposed to TKIs, GPR119 agonists potentiated the anticancer effects of TKIs.
ISSN
1756-9966
Language
English
URI
http://hdl.handle.net/10371/146960
DOI
https://doi.org/10.1186/s13046-018-0949-2
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College of Pharmacy (약학대학)Natural Products Research Institute(천연물과학연구소)Journal Papers (저널논문_천연물과학연구소)
College of Medicine/School of Medicine (의과대학/대학원)Nuclear Medicine (핵의학전공)Journal Papers (저널논문_핵의학전공)
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