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Silver nanoparticles promote procoagulant activity of red blood cells: a potential risk of thrombosis in susceptible population

Cited 10 time in Web of Science Cited 10 time in Scopus
Authors
Bian, Yiying; Kim, Keunyoung; Ngo, Thien; Kim, Inho; Bae, Ok-Nam; Lim, Kyung-Min; Chung, Jin-Ho
Issue Date
2019-02-14
Publisher
BioMed Central
Citation
Particle and Fibre Toxicology. 2019 Feb 14;16(1):9
Keywords
Silver nanoparticles (AgNP)ToxicityThrombosisRed blood cells (RBCs)Cancer
Abstract
Background
Silver nanoparticles (AgNP) are widely used in medical practices owing to their distinct antibacterial, antiviral and anticancer activities. However, with increasing use of AgNP, concerns over its potential toxicity are also escalating. Here, we demonstrated the potential thrombotic effect of AgNP which was mediated by the procoagulant activity of red blood cells (RBCs).

Results
In freshly isolated human RBCs, AgNP, but not silver microparticles (AgMP), elicited morphological changes, phosphatidylserine (PS) exposure and microvesicles (MV) generation, the key indicators of procoagulant activity in RBCs at concentration ranges (≤ 100 μg/mL) that were free of significant hemolysis. In line with this, AgNP potentiated thrombin generation and adherence of RBCs to endothelial cells, while AgMP did not. Oxidative stress, intracellular calcium increase and ATP depletion were found to underlie the procoagulant effects of AgNP, which led to altered activity of membrane aminophospholipid translocases. These in vitro findings were well reproduced in rat in vivo, where intravenously exposure to AgNP promoted venous thrombosis significantly. Of note, RBCs isolated from cancer patients, who inherently convey the risk of thrombogenesis, were more sensitive to the procoagulant effects of AgNP. In addition, AgNP significantly potentiated the procoagulant effects of a chemotherapeutic drug, paclitaxel.

Conclusion
Collectively, these results suggest that AgNP may have prothrombotic risks by promoting procoagulant activity of RBCs and caution shall be taken for its use in the population sensitive to thrombosis like cancer patients.
ISSN
1743-8977
Language
English
URI
http://hdl.handle.net/10371/147182
DOI
https://doi.org/10.1186/s12989-019-0292-6
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College of Pharmacy (약학대학)Dept. of Pharmacy (약학과)Journal Papers (저널논문_약학과)
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