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Reactive blue 2, an antagonist of rat P2Y4, increases K+ secretion in rat cochlea strial marginal cells

Cited 2 time in Web of Science Cited 3 time in Scopus
Authors
Lee, Jun Ho; Heo, Jeong-Hwa; Chang, Sun O; Kim, Chong-Sun; Oh, Seung-Ha
Issue Date
2006-07-15
Publisher
Elsevier
Citation
Hear Res. 2006 Sep;219(1-2):66-73. Epub 2006 Jul 12.
Keywords
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid/pharmacologyAdenosine Triphosphate/analogs & derivatives/pharmacologyAnimalsDose-Response Relationship, DrugElectrophysiologyEnzyme Inhibitors/*pharmacologyIon Transport/drug effects/physiologyPerilymph/metabolismPotassium/*metabolismRatsReceptors, Purinergic P2/agonists/*antagonists & inhibitorsSodium-Potassium-Exchanging ATPase/metabolismStria Vascularis/cytology/*drug effects/secretionSuramin/pharmacologyTriazines/*pharmacologyUridine Triphosphate/pharmacologyVibration
Abstract
Extracellular ATP decreases K+ secretion in strial marginal cells via apical P2Y4 receptors. We investigated the effect of reactive blue 2 (RB-2), an antagonist of rat P2Y4, on rat strial marginal cells using a voltage-sensitive vibrating probe. The application of RB-2 increased K+ secretion in a dose-dependent manner, and this increase was characterized as a peak followed by a partial relaxation to a steady-state. Moreover, this response was similar to that caused by 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS). Suramin had no similar effect, except at high concentration. Thus, we tested the effects of these chemicals on P2Y4 receptors in strial marginal cells. Both RB-2 and DIDS had antagonistic activities at P2Y4, and the antagonist potency at P2Y4 paralleled the potency of K+ secretion. Interestingly, 2'- and 3'-O-(4-benzoyl-benzoyl)adenosine 5'-triphosphate (BzATP) exhibited an agonistic effect at P2Y4 receptor, which was blocked by RB-2, but not by pyridoxalphosphate-6-azophenyl-2',4'-disulfonic acid (PPADS). Based on these results, we speculate that direct and/or indirect inhibitory mechanisms between P2Y4 and KENQ1/KCNE1 K+ channels exist in strial marginal cell.
ISSN
0378-5955 (Print)
Language
English
URI
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T73-4KCXJF1-6&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=cf4ad093796809579b070a3b08a29290

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16839719

http://hdl.handle.net/10371/14775
DOI
https://doi.org/10.1016/j.heares.2006.05.011
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Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Otorhinolaryngology (이비인후과학전공)Journal Papers (저널논문_이비인후과학전공)
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