SHERP

Sequential disruption of ALV host receptor genes reveals no sharing of receptors between ALV subgroups A, B, and J

Cited 0 time in webofscience Cited 0 time in scopus
Authors
Lee, Hong Jo; Park, Kyung Je; Lee, Kyung Youn; Yao, Yongxiu; Nair, Venugopal; Han, Jae Yong
Issue Date
2019-04-02
Publisher
BioMed Central
Citation
Journal of Animal Science and Biotechnology, 10(1):23
Keywords
Avian leukosis virusCRISPR/Cas9Genome editingHost receptoTVA
Abstract
Background
Previously, we showed that targeted disruption of viral receptor genes in avian leukosis virus (ALV) subgroups using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9))-based genome editing confers resistance to ALV subgroups B and J. Here, we used the same strategy to target the receptor expressed by ALV subgroup A (TVA) and generate chicken cells resistant to infection by this virus.

Results
CRISPR/Cas9-based disruption of exon 2 within the tva gene of DF-1 fibroblasts conferred resistance to infection by ALV subgroup A regardless of whether frameshift mutations were introduced during editing. Conversely, overexpression of the wild-type TVA receptor (wtTVA) by tva-modified DF-1 clones restored susceptibility to ALV subgroup A. The results confirm that exon 2, which contains the low-density lipoprotein receptor class A domain of TVA, is critical for virus entry. Furthermore, we sequentially modified DF-1 cells by editing the tva, tvb, and Na+/H+ exchange 1 (chNHE1) genes, which are the specific receptors for ALV subgroups A, B, and J, respectively.

Conclusions
Simultaneous editing of multiple receptors to block infection by different subgroups of ALV confirmed that ALV subgroups A, B, and J do not share host receptors. This strategy could be used to generate cells resistant to multiple viral pathogens that use distinct receptors for cell entry.
ISSN
2049-1891
Language
English
URI
http://hdl.handle.net/10371/153146
DOI
https://doi.org/10.1186/s40104-019-0333-x
Files in This Item:
Appears in Collections:
College of Agriculture and Life Sciences (농업생명과학대학)Research Institute of Agriculture and Life Sciences (농업생명과학연구원)Journal Papers (저널논문_농업생명과학연구원)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse